In total 3 vials BSA includedIncubation temperature Double Digests of Thermo Scientific restriction Incubate under paraffin oil in a capped vial Incubation at 37 ID: 849950
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1 1000 U Lot: ___ Expiry Date: _ï ï ï¯
1000 U Lot: ___ Expiry Date: _ï ï ï¯A A T Tï¯...3' 3'...ï ï ï¯T T A Aï...5' Concentration: 10 U/µL Supplied with: 1 mL of 10X Buffer B 1 mL of 10X Buffer Tango In total 3 vials. BSA includedIncubation temperature Double Digests of Thermo Scientific restriction Incubate under paraffin oil in a capped vial. Incubation at 37°C result
2 s in less than 10% activity. nuclease-f
s in less than 10% activity. nuclease-free water 16 µL 10X Buffer B 2 µL DNA (0.5-1 µg/µL) 1 µL TasI 0.5-2 µLPCR reaction mixture 10 µL nuclease-free water 18 µL 10X Buffer B 2 µL TasI 1-2 µLOverdigestion Assay on back page. stop the digestion reactipH 8.0 (#R1021), to achieve a 20 mM final extract with phenol/chloroform, precipitate with ethan
3 ol or isopropanol, wash the pellet with
ol or isopropanol, wash the pellet with 75% cold ethanol check the DNA concentration in the solution. CERTIFICATE OF ANALYSIS back page B GO R Tango2X Tango 20-50 0-20 20-50 0-20 pBR322 pUC57 pUC18/19 pTZ19R/UM13mp18/19 189 25 8 7 7 10 64 The ligation and recleavage assay was replaced with LO trace nuclease and phosphatase activities with sensiti
4 vity No detectable degradation of single
vity No detectable degradation of single-stranded or double-stranded labeled oligonucleotides occurred during PRODUCT USE LIMITATION The product was not tested for use in diagnostics or for drug Please refer to www.thermoscientific.com/onebio © 2012 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the ific Inc. and its subsidiari