PPT-Zhang Zhizhuo May 2010 Nucleosome

Positioning amp Transcription Factor Identification Outline Basic Concept Nucleosome Positioning and Gene Regulation General Transcription Mechanism Genomic Organization of Nucleosomes. : what, why and where?. Rob Brewster. Outline. What is a . nucleosome. ?. - how is DNA packaged/organized in Eukaryotes?. Why do . nucleosomes. form?. . - DNA is stiff, how do ~100 . bp. loops form so readily?. May 2010. Nucleosome. Positioning. & . Transcription Factor . Identification. Outline. Basic Concept. Nucleosome. Positioning and Gene Regulation. General Transcription Mechanism. Genomic Organization of . Y. Lyse & Sonicate. IP. Reverse crosslinks. Total. Reverse crosslinks. Amplify. Amplify. Sequence. Sequence. Immuno. -precipitation. Other controls for IP. (e.g., no antibody, non-specific antibody). A genomic code for . nucleosome. positioning. Eran. Segal, Yvonne . Fondufe-Mittendorf. , . Lingyi. Chen, . AnnChristine. . Thåström. , . Yair. Field, Irene K. Moore, . Ji. -Ping Z. Wang, and Jonathan . Correspondingauthor:FANLei,fanlei2004@yahoo.com.cnChinaNationalCommitteeforInternationalAssociationofMeteorologyandAtmosphericSciencesIAMASInstituteofAtmosphericPhysicsIAPandSciencePressandSpringer-Ve Forbes Jeffreys Zhang Ziyi , vol. 8, no. 1, January 2011.international cosmetics giant, Maybelline, and as an ResearchArticleSandplaytherapyAnoverviewoftheoryapplicationsandevidencebaseChristianRoeslerCatholicUniversityofAppliedSciencesFreiburgKarlsstr6379104FreiburgGermanyCorrespondingauthoratCatholicUnivers N3XXXXtBarndorff-NielsenandShephard2002Intheequidistantcaseiewhen1holdswithThemainintuitionisthattheItoprocesslocallyconditionallyGaussianandthusfeaturesakurtosiscoefcientequalto3Theequidistantcasecan 3D . genome organisation. Vladimir Teif. BS222 – Genome Science Lecture 6. Module structure. Genomes, sequencing projects and genomic databases (VT. ). Sequencing . technologies (VT. ). What is a gene? . Chromatin-binding factorshistone macromolecules that bind either directly or indirectly to DNA.Transcription factorhistone protein that directly binds to DNA.Architectural proteinsProteins that have a EpigeneticregulationofDNArepairgenesandimplicationsfortumorMarkusChristmann,BerndKainaDepartmentofToxicology,UniversityofMainz,ObereZahlbacherStr.67,D-55131Mainz,Germany Correspondingauthors.E-mailadd Rob Brewster. Outline. What is a . nucleosome. ?. - how is DNA packaged/organized in Eukaryotes?. Why do . nucleosomes. form?. . - DNA is stiff, how do ~100 . bp. loops form so readily?. Where do . Don and Ada . Olins. in 1974. , and their existence and structure (as . histone . octamers. surrounded by approximately 200 base pairs of DNA. ) were proposed by . Roger Kornberg. .. . The role of the nucleosome as a . Chromatin organization marks . exon-intron. structure. [Schwartz S, Meshorer E, Ast G . Nat . Struct. Mol . Biol. , 2009 Sep;16(9):990-6]. . Preface:. Crystal structure of . nucleosome. core particle.