PPT-7.3 Transcription in prokaryotes

731 State that transcription is carried out in a 5 3 direction Nucleotides are added in the form of ribonucleoside triphosphates 732 Distinguish between the sense and antisense strands of DNA. NusA. Stabilizes the RNA pol pausing. r. -independent termination. Figure 12.9. 2. Rho-independent terminator. . contains a short . inverted repeat. (~20 . bp. ). 3. Weakest base pairing: . A:U . make the dissociation easier. RNA Processing, and Transcriptional Regulation. Structure of RNA. Major Classes of RNA. Transcription in Prokaryotes. Transcription in Eukaryotes. Post-transcriptional Processing of Eukaryotic mRNA. Transcriptional Regulation in Prokaryotes:. Eubacteria & . Archarbacteria. Prokaryotes. Unicellular organisms that lack a nucleus. Prokaryotes are identified by characteristics such as shape, the chemical nature of their cell walls, the way they move, and the way they obtain energy.. Prokaryotic . Structure. size1-5. m. m (resolution of the human eye ~750. m. m). . Cell wall. . main component is peptidoglycan (modified sugar polymers). . can be further differentiated performing a gram stain . Single-celled organisms. Very tiny. Cannot be seen without a microscope. Our microscopes are not powerful enough. Lack a nucleus and most other organelles. Most numerous organisms on Earth. Extremely important organisms.. Structure of RNA. Major Classes of RNA. Transcription in Prokaryotes. Transcription in Eukaryotes. Post-transcriptional Processing of Eukaryotic mRNA. Transcriptional Regulation in Prokaryotes:. the Lac Operon as an example. GET READY TO USE THEM. STAND UP NOW . WITH CLICKERS IN HAND. WHEN I GIVE THE SIGNAL,. HOLD YOUR BREATH AS LONG AS YOU CAN.. I will count the time off.. When you can’t hold your breath any longer,. microscopic single celled organisms. collective biomass – . 10x of all eukaryotes!!!!!. vast genetic diversity among members. physical diversity. shapes: . spheres (. coccus. ), rods (bacilli) and spirals. . 1. The . trp. Operon. Contains 5 genes coding for proteins (enzymes) required for the synthesis of the amino acid tryptophan. Also contains a promoter and operator.. When tryptophan levels in the cell are low, the expression of the . Study Material for. . . B.Sc. Part II. . Botany . Hons. .. . . Paper IV. . Dr. Pushpanjali Khare. Sr. . What makes these two cell classes different?. Cells. The . cell. is the smallest unit of life in the universe.. ALL cells have two things in common:. They have . cell membranes. , which are barriers that separate the inside of a cell from the outside.. Classifying Prokaryotes. The smallest and most abundant microorganisms on Earth are . prokaryotes. —unicellular organisms that lack a nucleus. . Prokaryotes have DNA, like all other cells, but their DNA is not found in a membrane-bound nuclear envelope as it is in eukaryotes. Prokaryote DNA is located in the cytoplasm. . A eukaryotic transcription initiation complex consists of RNA polymerase II, . five. general transcription factors (GTFs), and a 20-subunit complex called Mediator (or . Srb. /Med). The CTD of RNA polymerase II anchors Mediator to the polymerase. Mediator allows RNA polymerase II to communicate with transcriptional . Professional medical transcription services provided by SpectraScribe offer a streamlined solution for researchers seeking to extract meaningful insights from various research methodologies.