PPT-ANIMAL CELL CULTURE

ANCHORAGE DEPENDENT CELLS Monolayer Substrate attachment for growth Charged substrate Contact inhibition controls growth Necessary for cells to spread or dividemitosis Determines the nature of the cell. Objective 34: I can describe the shape of an animal and plant cell.. Animal cells: are roundish shaped. Plant cells: are rectangular shaped. Objective 35: I can describe plant and animal cell organelles. WHAT IS CELL CULTURE ?. Cell culture . is the process by which cells are grown under controlled conditions, generally outside of their natural environment.. ADVANTAGES :. Development of cell line over many generations.. Part 2. Recovery of . Cells . from . Storage . Cells were stored in liquid nitrogen for a long time and need to be recovered for . experimental use. The vial should be immersed . in . a 37. o. C water bath and . . Technology. Ceac. 514. hıstory. Introduction. Cell culture is the process by which prokaryotic, eukaryotic or plant cells are grown under controlled conditions. . Practically. Cell . culture. is . introduction and history of acc . CELL CULTURE…... . In vitro culturing of animal cells, tissue fragment, organ or part of organ under aseptic condition using appropriate media….. ANIMAL TISSUE . PH.... optimum ph is 7.4.. Vary for different cell strain.. Plant tissue-5.5. Epidermal tissue-5.5. Transformed tissue-7-7.4. Fibroblast-7.4-7.7. Phenol red used as a indicator.. Temperature. ….. Depend on body temperature of animal from which cell is obtain.. We146re dedicated to providing you with speci31c cell culture needs and lab budget Tissue culture is the general name for the removal of cells, tissues or organs from an animal or plant and their subsequent placement into artificial environment conductive to growth. This environment usually consists of a suitable glass or plastic culture vessel containing a liquid or semi-solid support medium that supplies the nutrients essential for survival and growth. Cell Membrane. Cell Wall. Cytoplasm. Chloroplasts. Mitochondria. Nucleus. Vacuole. DRAW this table…. DRAW this table…. Plant Cell. Animal Cell. cytoplasm. chloroplast. vacuole. Cell. membrane. nucleus. Animal tissue culture. : is the growth of tissues separate from the animal . in vitro. (in the laboratory culture media. ).. Cell . line. Every cell present in the human body is not capable of growing in laboratory, only a few types of cells can grow . Dr. . Gatot. . Ciptadi. Lab. Of Genetics and Animal Breeding. Fac. of Animal Sci., . UB. LSIH-UB. BIOTEKNOLOGI REPRODUKSI . MAMPU MENYELAMATKAN. . SUMBER GENETIK. HEWAN . LANGKA. GT. Introduction. (Animal Biotechnology). Anil Gattani/Ajeet Kumar. Make . proteins:. commercial. scale. Stem. and . cancer. cells. CELL . CULTURE. Primary. Human. Antibody. production:. Why. . do. . it. . ?. and . . Saeed. Al-. Mossawi. Uses . Identification . Further studies . (e.g., Pathogenicity, antiviral sensitivity, research). Limitations . Absence of detection system for the agent. Inappropriate culture systems. Polyclonal and Monoclonal Antibodies (Catalog 1). Drug Screens , Animal Models and other Reagents (Catalog 2). Office of Technology Management. http://otm.uic.edu. /. . Catalog 2 Version: January 2013.