PPT-Nucleic Acid Extraction Control in Real-Time PCR Assays

Steve Hawkins Senior Global Product Manager Bioline RealTime PCR in Diagnostics Advantages Speed hours vs days Sensitivity Specificity Throughput Multiplexing Challenges False Positive Results. THE STUFF OF LIFE. Biomolecules. Organic molecules made up of:. Carbon. Hydrogen. Nitrogen. Oxygen. a. nd sometimes Phosphorus. LATIN 101. Poly = . Many. Mono = . O. ne. Hydro = . Water. . (actually Greek). Dr. . Sahar. Al . Shabane. Nucleic acids.  are biopolymers, or large . biomolecules. , essential for all known forms of . life. . Nucleic acids, which include . DNA.  (deoxyribonucleic acid) and . Consists of 5 carbon sugar, nitrogen base, and phosphate group. Repeating unit that makes up DNA, RNA, and ATP (energy for cells). What is a nucleic acid?. Store genetic material (DNA). Transfer genetic code (RNA). , a technique used to make numerous copies of a specific segment of . DNA.  quickly and accurately. The polymerase . chain reaction.  enables investigators to obtain the large quantities of DNA that are required for various experiments and procedures in . Shabane. Nucleic acids.  are biopolymers, or large . biomolecules. , essential for all known forms of . life. . Nucleic acids, which include . DNA.  (deoxyribonucleic acid) and . RNA. Each nucleic acid has its own unique sequence of bases that carries the genetic information from one cell to the next.. Learning . Goal . Describe the primary structures of DNA . and RNA. .. Nucleic Acid Structure. By:. . Preeti. . S. . Salve. KLE . College of Pharmacy, . Belagavi. A constituent Unit of KLE Academy of Higher Education and Research. Nehru Nagar, . Belagavi. . –. 590 010, Karnataka, India. Phone:. Pgs.:. 9-10. Heading: . Nucleic Acids. Homework:. . SILENTLY complete the Do First and glue on page 9. SILENTLY update notebook. Do First. DO FIRST (PG 9). Which biomolecule…. Makes up hair, nails, and feathers? c. Provides cells with cushioning. Real-Time PCR is a specialized technique that allows a PCR reaction to be visualized “in real time” as the reaction progresses.. This enables researchers to quantify the amount of DNA in the sample at the start of the reaction!. B2 - Compare the . structures. & . functions. of carbohydrates,. . lipids, proteins, & . nucleic acids . & . their organization into long complex molecules . Consist of:. . Carbon. , hydrogen, oxygen, . 1. Nucleic . Acids. Biochemistry 3070. Biochemistry 3070 – Nucleic Acids. 2. Historical Summary of the Discovery of DNA. The complexity of living processes require large amounts of information.. In the 19. & . Update on HCV . blood bank protocol. . Overview - . diagnostic tests. Serologic . assays: . specific antibody to hepatitis C virus (anti-HCV). Molecular . assays: HCV nucleic acid . (. nucleic. Download PDF Acid Reflux Solution Kit™ eBook by Barton - a comprehensive step-by-step system designed to treat acid reflux and heartburn issues naturally. - DNA & RNA. Types of Nucleic Acids. –. Virus with . ssDNA. Virus with . dsDNA. Virus with . ssRNA. Virus with . dsRNA. Virus with single stranded cyclic DNA. Virus with double stranded cyclic DNA.