PPT-Nucleic Acid Extraction Control in Real-Time PCR Assays

Steve Hawkins Senior Global Product Manager Bioline RealTime PCR in Diagnostics Advantages Speed hours vs days Sensitivity Specificity Throughput Multiplexing Challenges False Positive Results. Jesper Smith . IHMC. 40 South Alcaniz St . Pensacola. , . Florida 32502 . jsmith. @ihmc.us . Jerry Pratt. IHMC. 40 South Alcaniz St . Pensacola, Florida 32502 . jpratt@ihmc.us . Douglas Stephen. IHMC. THE STUFF OF LIFE. Biomolecules. Organic molecules made up of:. Carbon. Hydrogen. Nitrogen. Oxygen. a. nd sometimes Phosphorus. LATIN 101. Poly = . Many. Mono = . O. ne. Hydro = . Water. . (actually Greek). Automated real-time nucleic acid amplication technology for rapid and simultaneous detection of tuberculosis and rifampicin resistance: Xpert MTB/RIF assay for the diagnosis of pulmonary and extra - Dr. . Sahar. Al . Shabane. Nucleic acids.  are biopolymers, or large . biomolecules. , essential for all known forms of . life. . Nucleic acids, which include . DNA.  (deoxyribonucleic acid) and . 1-deoxyribonucleic acid (DNA). 2-Ribonucleic acid (RNA. Nucleic. Acids. Structure: Nucleotide. Monomer : . Nucleotide. A pentose sugar molecule .. 2.A phosphate group .. 3.A nitrogenous base .. Monomer : . Biomolecules. Organic molecules made up of:. Carbon. Hydrogen. Nitrogen. Oxygen. a. nd sometimes Phosphorus. LATIN 101. Poly = . Many. Mono = . O. ne. Hydro = . Water. . (actually Greek). Synthesis = . PCR activity: HiDi Taq DNA polymerase is tested successfully for hydrolysis probe based real - time PCR. The product demonstrates linearity of amplification over a specified serial dilution of HiDiTaq DNA polymerase istested successfully for hydrolysis probe based real-time PCR The product demonstrates linearity of amplification over a specified serial dilution of human genomic DNAThe activ Consists of 5 carbon sugar, nitrogen base, and phosphate group. Repeating unit that makes up DNA, RNA, and ATP (energy for cells). What is a nucleic acid?. Store genetic material (DNA). Transfer genetic code (RNA). Shabane. Nucleic acids.  are biopolymers, or large . biomolecules. , essential for all known forms of . life. . Nucleic acids, which include . DNA.  (deoxyribonucleic acid) and . RNA. Each nucleic acid has its own unique sequence of bases that carries the genetic information from one cell to the next.. Learning . Goal . Describe the primary structures of DNA . and RNA. .. Nucleic Acid Structure. Pgs.:. 9-10. Heading: . Nucleic Acids. Homework:. . SILENTLY complete the Do First and glue on page 9. SILENTLY update notebook. Do First. DO FIRST (PG 9). Which biomolecule…. Makes up hair, nails, and feathers? c. Provides cells with cushioning. Asst. . lec. . Mariam Ahmad Ali . Introduction to Viruses. Viruses are the smallest infectious agents (20 -300 nm in diameter), containing only one kind of nucleic acid (RNA or DNA) as their genome. The nucleic acid is encased in a protein shell, which may be surrounded by a lipid . Lecturer 7 . Dr. . Shaimaa. . Munther. . General. . Introduction. . Nucleic acids are required for the storage and expression of genetic information. . There are two chemically distinct types of nucleic acids: (.