PDF-Proc.Nati.Acad.Sci.USAVol.73,No.8,pp.2928-2931,August1976PhysiologyCyt

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ProcNatlAcadSciUSA731976292906OD05041mPMU03LDEPTHmmIIIIIIII00510FIG1AtracedcomputeroutputdisplayingtheaveragedopticaldensitiesoflayersinthespecimenofcerebralcortexshownbelowThescanni

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Proc.Nati.Acad.Sci.USAVol.73,No.8,pp.2928-2931,August1976PhysiologyCyt: Transcript

ProcNatlAcadSciUSA731976292906OD05041mPMU03LDEPTHmmIIIIIIII00510FIG1AtracedcomputeroutputdisplayingtheaveragedopticaldensitiesoflayersinthespecimenofcerebralcortexshownbelowThescanni. Proc.Natl.Acad.Sci.USA87(1990)6389Corey-Bransona-helixcannotberepresentedwithperfectaccuracyonanylatticeoflowcoordinationnumber,butthehelicesobservedincrystallographydatabasesofrealpro-teinsarelikewis Proc.Natl.Acad.Sci.USA87(1990)wereprepared(25),andtheDNAinsertwasexcisedwithEcoRI,isolatedbylowmeltagarosegelelectrophoresis,andrandom-primedforexpressionanalysis.Theexpressionlev-elsandsizesofthemRNA Proc.Nat.Acad.Sci.USA91(1994)9761thepGEM4vector(Promega)forsequencing.Specifically,a1.2-kbPstIfragment,whichhybridizedtothemousecDNAprobe,butnottotalmouseDNA,wassubclonedtogeneratethesequencehomologou MedicalSciences:Cherif-Zaharetal.Proc.Natl.Acad.Sci.USA87(1990)6245aatcccggcctgcacagagacggacacaggATGAGCTCTAAGTACCCGCGGTCTGTCCGG60MetSerSerLysTyrProArgSerValArg9CGCTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTC Proc.Natl.Acad.Sci.USA82(1985)855timeswiththesamebufferlackingNP-40andTritonX-100andwereresuspendedinthenick-translationbufferasde-scribedabovecontaining100,Ciof[3H]TTP(117Ci/mmol,NewEnglandNuclear)in Proc.Natl.Acad.Sci.USA83(1986)011CH2-C-COO-KETOACIDNH3+ICH2-CH-COO--OOCH20C0210II-CH-COO-IsolationofSpinachChloroplastsandPreparationofEx-tract.Chloroplastsfrom100gofspinachleaves,mid-ribsremoved,wer Proc.Natl.Acad.Sci.USA90(1993)2031dues,0.98forLexA-C52containingasinglecysteine,and0againforLexA-C52aftermodificationwith4-azidophenacylbromide,suggestingthatessentiallyallthecysteinesidechainshaverea Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby Cellsrespondtodevelopmentalcuesandenvironmentalstimulibyalteringgeneexpression.Often,transmissionofdifferentupstreamsignalsinvolvescommoncomponentsyetelicitsdistinct(butappropriate)outcomes.Numerousag Proc.Natl.Acad.Sci.USA93(1996)2583multiplexamplificationasdescribedabove.Theonlydiffer-encebetweenthesetwoproceduresisthatsomePprimersusedinMGSmaycontainsingle-basemismatchestothetemplatestoconvertthe Proc.Natl.Acad.Sci.USA88(1991)downstreamofthelacpromoterasfollows.PlasmidpHS202(5),whichcontainsnucleotides1-1010(thePvuII-KpnIchromosomefragmentthatencompassesruvA)intheSmaI-KpnIsiteofpUC19,wasdigest 2612Biochemistry:Sancaretal.PstIHaelilt*.JHinfl-TaqIadzJHpa11ti.r4HhaI'recAmRNA-recAprotein-Proc.Nati.Acad.Sci.USA77(1980)EcoRI-9A.-ii4------200020040060080010001200BasepairsFIG.1.Restrictionmapandseq

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