PPT-Using and Analyzing Fluorescent Proteins

Dina N Kovarik MS PhD Digital World Biology Updated April 24 2015 Fluorescent Proteins are Valuable Tools Locate proteins in the cell Track the migration of cells Reporter of expression Sister centromeres . By placing Cre under the control of a tissue specific promoter the Brainbow effect can be activated in virtual any tissue of choice This is called the R26R Confetti mouse In the following images provided by Dr Hugo Snippert R26R Confetti mice were b Germicidal Lamps Linear fluorescent andcompact fluorescent types Germicidal Applications UV lamps for germicidal applications.Water, air and surface sterilisation. Healthcare UVC is used to reduce the lamp (HCFL), flat fluorescent lamp (FFL), and light emitting diode (LED). Many kinds of LCD backlights as well as the characteristics of light sources for the backlights are addressed I. Introducti Fluorescence Technology. Josh Leung. James Weis. February 18th, 2010. Bio 1220, Gary Wessel. Fluorescence: How does it work?. Fluorescence vs Phosphorescence. -Time delay – microsecond vs min. Photon absorbed → photon released. with fluorescent proteins. Laboratory Introduction. What is a protocol?. What is . a protein?. Why do scientists . use protocols?. Why would we need to. purify proteins?. Now let’s. practice. pipetting!. Whole-body imaging with fluorescent proteins has been shown to be a powerful technology with many applications in small animals. Brighter, red-shifted proteins can make whole-body imaging even more s MULTI-LAYER DISC(FMD):. A Breakthrough in Optical. Data Storage. By-. GUNJAN CHAUDHARY. EC-3. rd. YEAR. N.I.E.C.. INTRODUCTION. In mid 1990’s,the company Constellation 3D ,in. New York City . demonstrates a new format: FMD (Fluorescent Multilayer Disk). Tyrannosaurus rex. Today’s Goal. Learn how a mass spectrometer can be used to identify the types of protein in a biological sample.. Learn how proteins are analyzed from a dinosaur fossil.. Be able to analyze protein mass spectra and to compare two mass spectra in order to identify an unknown protein fragment.. Tyrannosaurus rex. Today’s Goal. Learn how a mass spectrometer can be used to identify the types of protein in a biological sample.. Learn how proteins are analyzed from a dinosaur fossil.. Be able to analyze protein mass spectra and to compare two mass spectra in order to identify an unknown protein fragment.. Part 2: Comparing Fluorescent Proteins. 2013 Workshop C: Cloning DNA to Make Proteins. Dina N Kovarik, MS, PhD. NWABR. Fluorescent Proteins are Valuable Tools. Locate proteins in the cell. Track the migration of cells. 2018 1 Molecule of the Month Green Fluorescent Protein (GFP) A tiny fluorescent protein from jellyfish has revolutionized cell biology Green flourescent protein. The green fluorescent protein, shown : It is important to record the fluorescent image of the gel prior to EZBlue staining. EZBlue quenches the fluorescent signal. GlycoProfile III can also be used on PVDF membranes. After gel electroph Bioluminescent Reporters. Gene . Expression. Andrew Costello. Green Fluorescent Protein. Green Fluorescent Protein. First isolated in Aequorea victoria. Used to help identify certain proteins. Studies resulting from GFP research:. Since 2000, the RCSB PDB . Molecule of the Month. series has introduced millions of visitors to the shape and function of the 3D structures archived in the Protein Data Bank. The . Molecule of the Month.