the microscopic study of diseased tissue is an important tool in anatomical pathology since accurate diagnosis of many diseases usually requires histopathological examination of samples Histology Processing ID: 775367
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Slide1
Histology
Histopathology, the microscopic study of diseased tissue, is an important tool in anatomical pathology, since accurate diagnosis of many diseases usually requires histopathological examination of samples.
Slide2Histology Processing
Samples fix in 10% NBF.
48hrs -72 hrs -days
Slide3Paraffin infiltration and embedding into blocks.
Cuts thin sections on slides (3-5um thick).
Slide4Deparaffinization and Rehydration
Slide5Stain tissue sections
Hematoxylin and eosin stain (H & E stain)
structures staining blue are called basophilic because of their affinity for the basic dye (hematoxylin) structures staining pink are called acidophilic because of their affinity for the acid dye (eosin),
Slide6Liver
Hepatocytes
– functional liver cells
Hepatic Sinusoids
– small blood vessel within liver tissue
Hepatic Portal Vein
– brings blood into the liver
Central Vein
– takes blood out of the liver
Bile Duct
– storage and drainage of bile
Arteriole
– delivers blood from artery to capillaries
Glycogen Vacuole
– used for glycogen storage
Slide7Hepatic sinusoid
Hepatocyte
Glycogen
vacuole
Liver
1000X
Slide8Erythrocytes visible within the central vein
Hepatic sinusoids
Liver
400x
Slide9400X
Liver
Arteriole
Slide10RBC
Arteriole
Central vein
Liver
Slide11Liver
Bile Duct
Slide12Liver
Bile Duct
Slide13Liver
White Blood Cells
Slide14Head Kidney
Interrenal gland
– Is a cortisol producing tissue..
Chromaffin cell
– neuroendocrine cells that catalyze and secrete epinephrine, norepinephrine, and other hormones
Postcardinal vein
– Blood transport
Hematopoietic tissue
– where new blood cells are formed
Slide15A
B
400x
C
Interrenal Gland
Chromaffin Cell
Postcardinal vein
Head Kidney
Slide16Head Kidney
Chromaffin
cells
Cardinal
Vein
Interrenal cells
Slide17Trunk Kidney
Nephron – a renal tubule that removes waste and helps maintain homeostasisRenal corpuscleGlomerulus – Pressure filters blood plasma into Bowman’s capsuleBasement membrane – glomerulus membrane that does the blood filteringBowman’s capsule – collects filtered blood plasma and connects with proximal tubuleProximal tubule – selectively transports nutrients (amino acids, glucose, salt, ect.) back into the bloodDistal tubule – tubular secretion of molecules to balance pH Melanocytes – pigment cellsCollecting duct and/or Mesonephric duct – collects urea from multiple nephrons and controls futher water reabsorptionConnective tissue – fibres surrounding ducts
Slide18Proximal
tubules
Distal
tubules
Trunk
Kidney
Renal corpuscle
Slide19Glomerulus
Basement membrane
Bowmans
capsule
Trunk
Kidney
Slide20A
B
Mesonephric Duct
Melanocytes
Connective tissue
400x
C
Trunk
Kidney
Slide21Gills
Filament
– threadlike structure forming respiratory surface
Lamella
– fingerlike projections of the filament
Goblet (or mucous) cell
– secret mucus
Chloride cell
– acid/base regulation by Cl
-
and HCO
3
-
excretion
Cells surface area becomes enhanced during alkalosis and decreases during acidosis
Pillar (or pilaster) cell
– lend support for lamelle
Gill filament cartilage
– center of filament for structural support
Slide22Gill filaments and lamellae
Lamella
Filament cartilagePillar cellChloride cell
40x
A
B
C
Gill
Slide23Goblet
cells
Epithelial
cells
Gill
Slide24Pillar
cells
Erythrocytes
Chloride
cell
Gill
Slide25Laboratory Steps
Use proper microscope handling procedures (distributed in Lab 2)
Examine histology slides of liver, kidney, and gills
Examine additional histology slides of other organs