PPT-Gram Stain Differential stain (Hans Christian Gram, a Danish doctor ). He developed a

Differentiate bacteria into two large groups the Gram Positive and the Gram negative Gram status is important in medicine the presence or absence of a cell wall will change the bacteriums susceptibility to some antibiotics. Gram Stain Reaction. The Gram stain reaction, named after the Danish bacteriologist Hans Christian Gram, was originally devised in 1882.. Gram staining is based on the ability of the bacterial cell wall to retain the crystal violet dye. . Cheng . Zhang. Thurs 1 Dec 11. MM Tutorial. Gram staining. Fixed film (heat kill bacteria etc...). Methyl violet . Lugol’s. /gram’s iodine. Decolourise with acetone. Methyl red .  . STAIN 2 (counter stain): pink-red colour. Gram stain . Ziehl. . Neelsen. stain. . Requires the use of at least 3 chemical reagents.. Primary Stain. : . stain all the cells.. Decolorizing agent: . may or may not remove the primary stain from the entire the cell or only from certain cell structure. . Clinical Bacteriology II. CLS 413. Mrs. . Deemah. . Dabbagh. CLS Department. College of Applied Medical Sciences. King Saud University. Course Content. Enterobacteriacae. Lactose Fermenters:. Escherichia coli. Biology and Biotechnology department. Differential . stain separate bacteria into group . . Gram . Stain :. Differentiate bacteria into 2 groups:. Gram . positive bacteria . Gram . negative bacteria . Goals and Objectives. :. Exercise . 37. : Morphological Study of Unknown Bacterium. Each person make . smears of assigned organism: one from plate, one from broth (review smears Exercise 11). Gram stain (Exercise 15) and record results: Gram reaction, . Gram stain . Ziehl. . Neelsen. stain. . Requires the use of at least 3 chemical reagents.. Primary Stain. : . stain all the cells.. Decolorizing agent: . may or may not remove the primary stain from the entire the cell or only from certain cell structure. . Only use oil on 100x objective lens. Never get it onto any of the other objectives! . Never use the coarse adjustment with the 40x and 100x objectives.. . Ex. . 3: Staining Techniques - Gram Stain, part 1. Basic vs. acidic dyes. Differential Stain. Primary stain (stains all cells on slide). Decolorizing step (removes stain from certain types of cells). Counterstain (stains the decolorized cells). Differentiate bacteria into two large groups (the Gram Positive and the Gram negative).. Gram status is important in medicine; the presence or absence of a cell wall will change the bacterium's susceptibility to some antibiotics.. . By. Dr. . Shnyar. Hamid Qadir. Light Microscopy. Direct examination of stained or unstained preparations by . light (bright-field) microscopy. is particularly useful for detection of bacteria, fungi, and parasites.. & Mycobacterium. Bacterial shape. Bacilli. Cocci. Gram +ve. Gram –ve. Gram +ve. Neisseria spp.. Staphylococcus. Streptococcus. Gram -ve. Enterobacteriaceae. Spore forming:. 1. Bacillus spp. (aerobic). 1 The slide is washed in water; excess water is removed using a blotting paper, dried in air and heat fixed before observing under microscope. Those bacteria that hold on to primary dye-iodine comple 5/29/2023. 1. Practical Bacteriology. lab. . 2. By:. Asst. Prof. Shiama'a Al-Salihy. Learning objectives:. 5/29/2023. 2. After this lab, you must be able to:. Identify laboratory diagnosis steps.. Understand staining techniques.