Alison Holiday Alzheimers Disease AD Leptin Leptin 116130 prevents copper induced cell death Crystal violet assay on leptin and leptin 116130 treated cells with copper CuCl 2 treated ID: 913763
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Slide1
Investigating a Fragment of the Leptin C-D loop: Neuroprotective and Behavioural Effects
Alison Holiday
Slide2Alzheimer’s Disease (AD)
Slide3Leptin
Slide4Leptin116-130 prevents copper induced cell death
Crystal violet assay on leptin and leptin
116-130
treated cells with
copper
CuCl
2
-treated
cultures
CuCl
2
-treated
cultures
Slide5Leptin116-130 prevents amyloid beta induced cell death
A
b
1-42
-treated
cultures
***
**
*
LDH assay on leptin and leptin
116-130
treated cells with amyloid beta
A
b
1-42
-treated
cultures
***
**
*
Crystal violet assay on leptin and leptin
116-130
treated cells with
amyloid beta
Slide6Leptin116-130 acts via STAT3 and Akt pathways
**
**
Ratio of phosphorylated-STAT3 to pan-STAT3 in protein extracts from untreated and leptin
116-130
pre-treated cells
Ratio of phosphorylated-
Akt
to pan-
Akt
in protein extracts from untreated and leptin
116-130
pre-treated cells
Slide7Leptin
116-130
improves episodic memory
Object-place-context recognition test
Healthy C57 mice injected with saline, leptin or leptin
116-130
fragment
Discrimination
index
=
Novel - Familiar
Novel + Familiar
Slide8Leptin116-130 improves episodic memory
Total exploration times for mice in each treatment group
Discrimination index for mice in each treatment group
Slide9Slide10Conclusion
Slide11Acknowledgements
Collaborators from Dundee:Dr Jenni HarveyYasaman
Malekizadeh
Supervisors:
Dr Gayle Doherty (
https://doherty-lab.wp.st-andrews.ac.uk/
)
Dr James Ainge
Devon
Redfearn
Publication: Malekizadeh, Y., Holiday, A., Redfearn
, D., Ainge, J. A., Doherty, G. and Harvey, J. (2016) A Leptin Fragment Mirrors the Cognitive Enhancing and Neuroprotective Actions of Leptin. Cerebral Cortex, In press.
Slide12Questions