yea MANALOT an HAYES 11 HAYE e al 1989 reporte dengu viru infecti - PDF document

yea (MANALOT an HAYES [11]) HAYE e al (1989 reporte dengu viru infection i 2America militar personne i 1984 Anothe stud wa conducte fro May 198 t January 198 i a hospita i Manila wher 51 patient wer foun t b infecte b denguviruses Dengu 2 wa th predominan serotype followe b Dengu 3 an Dengu 1 respectively Dengu 4 wa foun onl i a fe isolate (HAYE e al [8]) A tota o 37 Filipinchildre ou o a tota o fro October 198 t March 198 wer foun thav dengu feve o dengu hemorrhagi feve e al [13]) Fe studie hav showtha Japanes Encephaliti doe occu i th Philippine wit th majorit o th case affectin th on t te yea ol ag grou i place wher ric field abound Accordin t th autho th morbidit rat i 1 - 1796 wit a mortalit rat o abou 7 - 30 (BARZAG [1])Unti toda al investigation o th activit o th tw arboviruse wer conducte i anaroun Manila th capita o th Philippines an o a fe othe place o th mai islan oLuzon Ou presen stud o Mindoro a islan clos t Luzo shoul demonstrat th presenc o dengu an J virusesMateria an Method0. m o untreate seru specime wer obtaine fro outpatient visitin th hospita fodifferen reasons mos o the ha fever Seru collectio starte i Novembe 199 anwa finishe i March 1992 Th froze ser wer store i Calapa unti dispatc t ViennaI orde t avoi unspecifi reactions th ser wer treate wit aceton an afterward teste i a dilutio o 1 agains 4 t 8 antige unit o Dengu 2 an 4 an als o thhemagglutinatio inhibitio (HI tes (CLARK an CASAL [4] i U-Nunc-Microtiterplates Furthe dilution wer don geometricall tResult an Discussio4 o a tota numbe o 12 ser teste wer no reactiv wit on o th thre antigens 4ser reacte pos

itiv agains D an D antigens Mos o th ser showe th sam titeragains bot antigens i som case th titer agains D wer highe tha thos D4 3 ser wer positiv agains J (Tab 1)Man seru specimen showe cros reaction i th H tes betwee D2/D an Jantigen Bette result wer obtaine b BURK e al (3 usin epitope-blockin immunoassayfo antibod detectio i huma sera Th author foun th result superio t th H tes comparabl t th plaqu reductio neutralizatio fo identifyin subject immun t Dan J o bot viruses Antibod respons i J an DH patient wa measure b th indirec micr ELIS b BUND e al (2) Th author reporte tha response o rathemonospecifi i contras t DH case whos antibod response wer cross-reactiv t Jan D antigen eve i th primar infection GUNASEGARA e al (7 develope a highl specific an economica hemadsorptio immunosorben techniqu fo th detectio odengue-specifi immunoglobuli M (IgM antibody Th techniqu i base o th reactio o ser wit anti-huma Ig immobilize ont a soli phas followe b th detectio o Ig b th additio o a know quantit o dengu viru hemagglutini an erythrocytes Dengue-specifi Ig di no interfer wit th results no wa ther ancross-reactivit betwee dengue-hemagglutinin an Ig specifi fo othe virusesJo an WAD (10 reporte o prevalenc o th vecto mosquitoe o Korea Thecoul demonstrat th rol o thre specie o culicid mosquitoes Culex tritaeniorhynchus,C. pallens, an Anopheles sinensis, transmittin JE Th seasona pea occurrenc oth thre specie wa differen a wel a th night-tim bitin activity iprobabl th mos importan vecto o J viru i towns an both C. tritaeniorhynchus anC. pallens woul b importan i rura localities OLSO e al (1

2 isolate J viru froAnopheles annularis an A. vagus i Lombok Indonesia Th frequencie o infectio180 Tabl 1Antibod titer o th 8 positiv serDilutio 1Dengu 2 an 4JapanesEncephaliti225485815161732316424128 tota42 3i zoophili Anophele specie wa highe tha iC. tritaeniorhynchus, th principa vecto o Jviru i Asia Accordin t th author th lofrequenc o infectio i C. tritaeniorhynchus anth relativel infrequen raisin o pig (i aIslami country ma accoun fo th lo prevalenc o J neutralizin antibodie i th humapopulation o LombokTh natura foc o J viruse ar towno th on hand an rura area o th othehand Differen vecto specie transmi th virusesTh mai vecto o th urba cycl o i Aedes aegypti, i rura area othe membero th subgenu Stegomyia o th genu Aedes transmi th D virus i e A. albopictus an thA. complex J viru i mainl transmitte b C. tritaeniorhynchus i urba a wela i rura areas A larg numbe o othe specie o culicin an anophelin mosquitoe arals involve i th natura cycl o th virus a ca b see above Th vector o bot viruse ar ric padd breedin mosquit specie wit a marke preferanc fo anima feedingFiel trial wit experimentall develope liv vaccine wer conducte i Thailanagains D virus Som vaccine agains J viru hav bee availabl fo man year an arproduce mainly i Japan DENNIN an KANEK (6 sugges tha al visitor t rura areashoul receiv vaccinatio agains JE particularl durin th summe monsoo month iSoutheas AsiaSummar 12 huma seru specimen wer obtaine fro th Provincia Hospita i Calapan OrientaMindoro i orde t gathe informatio abou th occurrenc o Dengue Dengu Hemorrha Feve an Japanes Encephaliti i Orienta Mindoro I th hemagglu

tinatio inhibitiotes 4 ser wer negative 4 positiv agains Dengu virus an 3 positiv agains JapanesEncephaliti virusKe word Dengue Japanes Encephaliti viruses Mindoro/PhilippinesZusammenfassunVerbreitung von Dengue und Japan B-Enzephalitis inOst-Mindoro, Philippinen12 Serumprobe au de Provinz-Spita i Calapan Ostmindor wurde au de Gehal aAntikörper gege Dengu (Ty 2 un 4 un Japonic B Enzephaliti untersucht I Haemagglutinationshemmungs-Tes zeigte sic 4 Probe negati gege beid Antigene 4 Probezeigte positiv Tite gege Dengue-Vire un 3 solch gege Japonic B Enzephaliti VirusSchlüsselwörte Dengu Virus Japa B Enzephaliti Virus Mindoro/PhilippinenReferenceBARZAGA N G (1989)A revie o Japanes encephaliti case i th Philippine (197 - 1985)Southeas Asia J Trop Med Publ Health 2 (4) 587-592BUNDO K. TORRES C A. CHANYASANHA Ch LINN M L 1GARASHI A (1986)Antibod respons i Japanes encephaliti an dengu hemorrhagi feve patient measureb indirec EL1SATrop 2 (2) 101-114181 3Detectio o Flaviviru antibodie i huma seru b epitope-blockin immunoassayJourna o Medica Virolog 2 (2) 165-1744 D H. CASALS J (1958)Technique fo hemagglutinatin an hemagglutinatio inhibitio wit arthropo born virusesAmer J Trop Med Hyg 7 561-5735 MADRID A T. PORTERF1ELD J S (1974)Th flaviviru (grou B arboviruses) a cross-neutralisatio studyJ Gen Virol 23 91-966 DENNING D W. KANEKO Y (1987)Shoul traveller t Asi b vaccinate agains Japanes encephalitisLancet 853-8547 K. LIM T H. AHMED J G. AASKOV J G. LAN K. PANG T (1986)Hemadsorptio Immunosorben Techniqu fo th Detectio o Dengu Immunoglobuli M AntibodyJourna o Clinica Microbiolog 2 (1) 170-17

48 HAYES C G. MANALOTO C R. GONZALES A. RANOA C P (1988)Dengu infection i th Philippines clinica an virologica findings i 51 hospitalize patientsAmer J Trop Med Hyg 3 (1) 110-1169 HAYES C G. O'ROURKE T F. FOGELMAN V. LAEVENGOOD D D. CROW G. ALBERSMEYER M M (1989)Dengu feve i Americ militar personne i th Philippines clinica observation o hospitalize patientdurin a 198 epidemicSouther Asia J Trop Med Publi Healt 2 (1) 1-810 C Y. WA A Y (1985)Seasona prevalenc o th vecto mosquito o Japanes encephaliti viru i Kyungpoo Province KoreaKorea Journa o Parasitolog 2 (1) 139-1501 MANALOTO C R. HAYES C G (1989)Isolatio o dengu viruse fro hospitalize patient i th Philippines 198 - 1986Southeas Asia J Trop Med Publ Healt 2 (4) 541-54712 J G. KSIAZEK Th G. LEE V H. TAN R. SHOPE R E (1985)Isolatio o Japanes Encephaliti Viru fro Anophele annulari an Anophele vagu i Lombok IndonesiaTransaction o th Roya Societ o Tropica Medicin an Hygien 79 613 R S. HAYES C G. LEUS S D. MANALOTO C O (1987)Dengu fever/dengu haemorrhagi feve i Filipin children clinica experienc durin th 198 - 198epidemicSoutheas Asia J Trop Med Publ Healt 1 (3) 284-290Korrespondenzadresse A RaddInstitu fü Virologi de Universitä WieKinderspitalgass 1A-109 Wie • Austri182 Mitt Österr GesTropenmed Parasitol 1 (199317 - 18Institu o Virolog (Director Ch Kunz o th Universit o Vienna Austri (1Provincia Hospital Calapan Orienta Mindoro Philippine (2Occurrence of Dengue and Japanese EncephalitisViruses in Oriental Mindoro, PhilippinesA C Radda Teres G Escalona Heid DippeIntroductio Dengu an Dengu Hemorrhagi Feve ar cause b viruse o th