PPT-Fluorescence Polarization

Djoshkun Shengjuler JOJO Cameron Lab Feb 25 th 2015 PSUBMB Outline History Measurement Principle AdvantagesDisadvantages Applications Case studies 3 Special Considerations History. AIT. Exercise . 2.1. Atomic. Molecular. Absorption. Emission. flame AAS. UV/VIS. IR. flame emission. ICP. ??. Fluorescence. Interaction with radiation. absorption to exc. state via heat, electricity, radiation. . What is a . fluorescence. ?. Wiki. : emission of light by a substance that has absorbed light or other electromagnetic radiation of a different wavelength.. The name coined by George Gabriel Stokes in 1852 “. 1. Dr. Nikhat Siddiqi. Principles. Interaction of photons with molecules results in promotion of valence electrons from ground state . orbitals. to high energy levels.. The molecules are said to be in excited state.. . a . semi-analytical ocean color model for MODIS and beyond. Stéphane. . Maritorena. & Dave Siegel. Earth Research Institute. University of California, Santa Barbara. - . Develop. a . fluorescence . 20406080100 mP 100150200300 , J. Tres Brazell*, Carsten Schwerdtfeger*, Farid El Oualid, Huib Ovaaand Francesco Melandri**Boston BiochemInc., Cambridge, MA 02139, UbiQBio BV Amsterdam, the Netherlands FPIA. Fluorescence Polarization Immunoassay. FPIA. Fluorescence polarization . is ideal for the study of small molecule fluorescent . ligands. binding to receptors.. In fluorescent polarization method of assay, polarized light excites a fluorescent label. As this label is bound, the degree of polarization of the fluorescent emission from the label increases. . FPIA. Fluorescence Polarization Immunoassay. FPIA. Fluorescence polarization . is ideal for the study of small molecule fluorescent . ligands. binding to receptors. .. In . fluorescent polarization method of assay, polarized light excites a fluorescent label. As this label is bound, the degree of polarization of the fluorescent emission from the label increases. . Carma. probing small-scale magnetic fields in star-forming regions. Chat Hull. University of California, . Berkeley. Radio Astronomy Laboratory. 10 April 2013. Special Session: Polarization. ALMA 2013 Rocks!. KAARTHIK J. INTRODUCTION. A fluorescence microscope is a optical microscope that uses fluorescence and phosphorescence instead of , or I addition to reflection and absorption to study properties of organic or inorganic substances.. Markus . B. öttcher. North-West University. Potchefstroom, South . Africa. 0. Most of this work done by:. Haocheng. Zhang. . (Ohio University & Los Alamos). Leptonic. . Blazar. . Model. Relativistic jet outflow with . How did 1. st. week of labs go? . Questions? Comments?. Polarization assays—important for . k. 2. and for binding assays (Lab 2). Fluorescence Polarization. Important for binding assays. Also important for . It is a phenomenon of emission of radiation when the molecules are exited by radiation at certain wavelength.. FLUORIMETRY:-. It is measurement of fluorescence intensity at a particular wavelength wit the help of a filter fluorimeter or a spectrofluorimeter.. of a . fluorophore. FLT does not depend on . fluorophore. concentration, absorption by the sample, sample thickness, method of measurement, fluorescence intensity, photo-bleaching, and/or excitation intensity. It is affected by external factors, such as temperature, polarity, and the presence of fluorescence quenchers. Fluorescence lifetime is sensitive to internal factors that are dependent on . and its diffusion in biological liquid flow. Maryakhina. V.S., . Gun’kov. v.v.. Orenburg state university. v.s.maryakhina@gmail.com. Exemplary fluorescence images of one animal after injection 2 .