PPT-Fluorescence Polarization

Djoshkun Shengjuler JOJO Cameron Lab Feb 25 th 2015 PSUBMB Outline History Measurement Principle AdvantagesDisadvantages Applications Case studies 3 Special Considerations History. AIT. Exercise . 2.1. Atomic. Molecular. Absorption. Emission. flame AAS. UV/VIS. IR. flame emission. ICP. ??. Fluorescence. Interaction with radiation. absorption to exc. state via heat, electricity, radiation. Chelsea Aitken. Peter Aspinall. Advantages Over Light Microscopy. Resolution of light microscopy limited by Rayleigh Criterion. If two objects cannot be seen as distinct structures, then they may be considered coincident in space. . What is a . fluorescence. ?. Wiki. : emission of light by a substance that has absorbed light or other electromagnetic radiation of a different wavelength.. The name coined by George Gabriel Stokes in 1852 “. . M. . Uherek. 1. , J. . Fischerova. 2. . and. . D. . Chorvat. . jr. .. 1,2. 1. International Laser Centre, Bratislava, Slovakia . 2. Faculty of Mathematics, Physics and Informatics, Comenius University,. Jeffrey Hatef. Mentor: Dr. Alan . Kogut. Inflation Theory: How is the Universe so big?. The universe appears too big, inflation is a possible answer.. COBE and WMAP. COBE beginning in 1989 and WMAP in 2001 have mapped the sky in the microwave range.. Lyes. . Lakhal. Institut Polytechnique . LaSalle. Beauvais. Rue Pierre WAGUET. BP 30313. F-60026 BEAUVAIS . Cedex, France.. . Workshop on Tensor Decompositions and . Applications. , . 2010. Sept. 13-17, 2010, Monopoli, Bari, Italy. Chris Packham. University of Texas at San Antonio. Outline. Polarization processes of importance to AGN. Scattering. Dichroism. Synchrotron. Sy’s. and BLs. MHD & the torus. Conclusions. Caveats: This review talk is being far from comprehensive, and rather focuses on some topics that I’ve enjoyed reading of late – sorry to all those I offend by not featuring their great work. FPIA. Fluorescence Polarization Immunoassay. FPIA. Fluorescence polarization . is ideal for the study of small molecule fluorescent . ligands. binding to receptors.. In fluorescent polarization method of assay, polarized light excites a fluorescent label. As this label is bound, the degree of polarization of the fluorescent emission from the label increases. . FPIA. Fluorescence Polarization Immunoassay. FPIA. Fluorescence polarization . is ideal for the study of small molecule fluorescent . ligands. binding to receptors. .. In . fluorescent polarization method of assay, polarized light excites a fluorescent label. As this label is bound, the degree of polarization of the fluorescent emission from the label increases. . Chlorophyll Concentrations . of Vegetables. Jane Kim & Rebecca Long. Ch251B – Instrumental Analysis Lab. Professor A. . Newmark. Background – Why Chlorophyll?. Health benefits in chlorophyll:. Toby K. Westberry, Michael J. Behrenfeld . With (lots of) help from. Emmanuel Boss, Allen Milligan, Dave Siegel, Chuck McClain, Bryan Franz, Gene Feldman, Scott Doney, Ivan Lima, Jerry Wiggert, Natalie Mahowald, others. Dr.. A. K. Dhingra. Indocyanine. . G. reen Dye - ICG. w. as . developed for near- infrared (NIR) photography by . Kodak Research . Labs. . in 1955 and was introduced in clinical practice . in . 1956 . How did 1. st. week of labs go? . Questions? Comments?. Polarization assays—important for . k. 2. and for binding assays (Lab 2). Fluorescence Polarization. Important for binding assays. Also important for . and its diffusion in biological liquid flow. Maryakhina. V.S., . Gun’kov. v.v.. Orenburg state university. v.s.maryakhina@gmail.com. Exemplary fluorescence images of one animal after injection 2 .