PPT-Purification

Introduction to the research information platform PURE Ann Verlinden Dirk Schoonbaert Nico Van Aerde 11 June 2014 The concept and importance of PURE Features Roles and responsibilities Personal overview. 43. Copyright © McGraw-Hill Global Education Holdings, LLC. Permission required for reproduction or display.. Water Purification and . Sanitary Analysis. Microbial . containment candidates. potential pathogens that can survive in water and represent severe health risks. Ian Gluck. Mentor: Dr. Christine Kelly. OSU Dept. of Chemical, Biological and Environmental Engineering. The Biofuel Industry. The Conversion Process. Cellulose must be converted to glucose. Performed by cellulases. Course: Methods in protein chemistry. Rahman M. . Mahfuz. ur. 2012/01/11. SLU. To seperate a . particular. protin from all other proteins and cell . components. . There are many types of proteins within an ogranism . HIC Chromatography. Chromatography. A technique used to separate molecules based on how they tend to cling to or dissolve in various solids, liquids, and gases.. Characteristics of Proteins. Hydrophilic. A Facility for Materials selection ... Randoms. .. 1/11/2013. Instrumentation Frontier Meeting at ANL - S. Pordes . 1. 1/11/2013. Instrumentation Frontier Meeting at ANL - S. Pordes . 2. My hands-on experience is limited to liquid Argon aimed at Neutrino Detectors... Plasmid . What is the plasmid ?. Plasmid are small, double strand , closed circular DNA molecules , that can be isolated from . bacterial cells. , they replicate independently of the bacterial chromosome. 2. . AGELOC OPPORTUNITY . LAUNCH KIT—. PHASE. TWO. *View notes section for more information. SECTION ONE. ageLOC. R. 2. PRODUCT OVERVIEW. Are you ready to feel and live younger? . To remind your body to act young again?. Proposing a Procedure. Library. Primary literature. SciFinder. Scholar. Secondary Literature. Organic Syntheses. Fieser. Comprehensive Organic Transformations. Case Study: Benzoic Acid. Literature: Try a Grignard Reaction. Part I. IUG, Fall 2012. Dr. . Tarek. . Zaida. 1. Background. LDH catalyzes the . nicotinamide. cofactor-dependent . interconversion. of lactate and . pyruvate. :. 2. LDH is found in almost all organisms because it plays an important role in carbohydrate metabolism during conditions in which . Iman . Alshehri. Introduction . Iman . Alshehri. In this course, you will use lactate dehydrogenase (LDH) as the subject of . your studies. . LDH (. E.C. . 1.1.1.27) catalyzes the nicotinamide . cofactor-dependent. Introduction. The . organization of this presentation includes. :. . I.. . Basic methods of . Isolation . and purification . . II.. . Special Methods of purification:. a). Counter-current Extraction. NBC2 . Biomanufacturing Steps in . Product Purification. UPSTREAM PROCESSING. . Genetically . engineered protein produced by . an organism (ex. E.coli) are grown to yield the optimal amount of product. TRIzol. purification: precipitate out everything BUT the RNA. Two common methods for separation of RNA from DNA. Selective precipitation of RNA using Lithium Chloride (LiCl). Extraction with phenol buffered at an . Many cloning vectors have been engineered so that. the protein being expressed will be fused to another. protein, called a . tag, which can be used to facilitate. protein purification. Examples of tags include glutathione-.