Hopkins Microbiology Course Ann Lesnefsky 7152011 Metabolism of PNS Bacteria Organic acid electron donor Photosynthesis for energy generation Bacterial chlorophyll b ID: 376866
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Slide1
Purple Non Sulfur Bacteria
Hopkins Microbiology Course
Ann
Lesnefsky
7-15-2011Slide2
Metabolism of PNS Bacteria
Organic acid electron donor
Photosynthesis for energy generation
Bacterial chlorophyll b
Absorbs light in the infrared range
Currently PNS bacteria are being studied for H2 productionSlide3
Course Enrichment Method for PNS
6-18-2011
Plated samples from the slough on succinate and ethanol plates,
incubated
anaerobically with light
Course’s media and Holly’s media6-29-2011Restreak single colonies for isolation Slide4
TA Enrichment Method for PNS
TA’s enriched for PNS by filtering
Filter both water above mat and slurry of mat material
Place filter face down on plate
Flip and all allow PNS bacteria to grow on filterSlide5
Carbon SourceEnrichment Method
Succinate
(# of
colonies
before
restreak)
Ethanol
(# of
colonies
before
restreak
)
No filter
Course
43 6Holly 0 0 FilterCourse Many SomeHolly Few NoneSlide6
Microscopy of PNS
Colonies isolated from 5 different plates
4 plates from strawberry
1 from
Kerby
Park
All looked like stubby rodsSlide7
Recommendations
Next year use the filter method.Slide8
Questions?