PDF-28.F.Desdouits,J.C.Siciliano,P.Greengard,J.A.Girault,Proc.Natl.Acad.Sc

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firstbecameinterestedinthestudyofmemoryin1950asaresultofmyreadingsinpsychoanalysiswhilestillanundergraduateatHarvardCollegeLaterduringmedicaltrainingIbegantofindthepsychoanalyticapproachlimitingb

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28.F.Desdouits,J.C.Siciliano,P.Greengard,J.A.Girault,Proc.Natl.Acad.Sc: Transcript


firstbecameinterestedinthestudyofmemoryin1950asaresultofmyreadingsinpsychoanalysiswhilestillanundergraduateatHarvardCollegeLaterduringmedicaltrainingIbegantofindthepsychoanalyticapproachlimitingb. Proc.Natl.Acad.Sci.USA90(1993)8129Stimulusaz0C.)ww-JcnnNeuron'sResponseFourierFundamentalsAverage*Response=;;~~~~~~Noise=N-RiREALFIG.1.Calculationofaganglioncell'saverageresponseandresponsenoiseto10cy Proc.Natl.Acad.Sci.USA74(1977)4133.(iii)abFIG.5.Thepackingoftwoindependentpleatedsheets.(a)Twopleatedsheetsarerepresentedbytwothinsmoothsheets.Arrowsindicatethechaindirection.Eachsheethasaright-handed Proc.Natl.Acad.Sci.USA90(1993)10391days.Whenthecellswereforamicroinjectionexperiment,coverslipswerefirstmarkedwithadiamond-tippentofacilitatethelocalizationandvisualizationofmicroinjectedcells.After8- Proc.Natl.Acad.Sci.USA90(1993)RESULTSElectronMicroscopyofPNA-DNAComplex.Weuseda(dA)98-(dT)98targetcontainedwithinasupercoiledplasmid,pA98,linearizedwiththeCfrlOIrestrictionenzyme,andchallengedthiswith Proc.Natl.Acad.Sci.USA92(1995)7141FRETefficiencyRNAbulgeA7AgFIG.2.KinkingofDNAandRNAduplexesbybasebulgesisrevealedbyFRET.Thedistancebetweendonor(D)andacceptor(A)fluorophoresattachedtothe5'terminiofthe Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl Proc.Natl.Acad.Sci.USA92(1995)FIG.1.CytologyofDrosophilafemalemeiosis.Theimagesareconfocalmicrographsofoocytesfixedandlabeledwithantibodiestovisualizemeioticstages.(A)MetaphaseIarrestinamaturestage14o Proc.Natl.Acad.Sci.USA93(1996)11529BothLandL*canbeprotonatedonLys-156,butthepKaforprotonationisconsiderablylowerforL*thanforL;pKL*iswhilepKLis10.OneimportanttenetofthismodelisthattheIndsmutationsincre Proc.Natl.Acad.Sci.USA90(1993)2031dues,0.98forLexA-C52containingasinglecysteine,and0againforLexA-C52aftermodificationwith4-azidophenacylbromide,suggestingthatessentiallyallthecysteinesidechainshaverea I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 Proc.Natl.Acad.Sci.USA93(1996)2583multiplexamplificationasdescribedabove.Theonlydiffer-encebetweenthesetwoproceduresisthatsomePprimersusedinMGSmaycontainsingle-basemismatchestothetemplatestoconvertthe Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby Proc-NatLAcadSciUSA9019938019co11rICSCNSMPIass-x0000Isi-2W4T-jINkIIIIIIIIII2NcP-oo0NPI000Nrs0aNooNoNNNCM0JwtNaICYiiID-iNccerco000os9zSzE3oSZlilEANwlMkIICCDON000oNVnoCD0cotNNNN--IICOCYwvrw5oIcasNecUo02

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