PDF-Proc.Natl.Acad.Sci.USA74(1977)4131FACEUPbCLASSIi

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ProcNatlAcadSciUSA7419774133iiiabFIG5ThepackingoftwoindependentpleatedsheetsaTwopleatedsheetsarerepresentedbytwothinsmoothsheetsArrowsindicatethechaindirectionEachsheethasarighthanded

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Proc.Natl.Acad.Sci.USA74(1977)4131FACEUPbCLASSIi: Transcript

ProcNatlAcadSciUSA7419774133iiiabFIG5ThepackingoftwoindependentpleatedsheetsaTwopleatedsheetsarerepresentedbytwothinsmoothsheetsArrowsindicatethechaindirectionEachsheethasarighthanded. Proc.Natl.Acad.Sci.USA90(1993)RESULTSElectronMicroscopyofPNA-DNAComplex.Weuseda(dA)98-(dT)98targetcontainedwithinasupercoiledplasmid,pA98,linearizedwiththeCfrlOIrestrictionenzyme,andchallengedthiswith Proc.Natl.Acad.Sci.USA90(1993)424Age(months)200.0120z0.010laaCD-_-0.008oo100CQ0.006coCD~0o0.004x0NuclearDNATI0.002F0.04242a100000Q0la)co0x0Age(months)FIG.1.Steady-stateoxidativeDNAdamageincreaseswitha Proc.Natl.Acad.Sci.USA83(1986)3195U3R[U311PUvir5DNALTR-RUSU+ViralRNABamHIXhoILTRydLTPrototypePIli3400(3200)NheINheILEZ3EIILIZ3~MTZIJL*3200NT0,2200NT-SVNEO*3000NT*2200NTMT-NBLTRdLTRSV-N"1C7272U3NheIPvu Proc.Natl.Acad.Sci.USA93(1996)9293exonjunctionsinthespy-1andspy-2mutants,genomicDNAwassubjectedtoPCRandtheproductsweresequenceddirectly.RESULTSANDDISCUSSIONIsolationofspy-4.Thespy-4mutantwasisolatedby Proc.Natl.Acad.Sci.USA90(1993)solvedinapyrogenicwaterandthesolutionwasfilteredthrougha0.22-,mcelluloseacetatefilter.Thelaminarinfiltratewaslyophilizedandresuspendedtoaconcentrationof1.47mg/ml.Lipopoly Proc.Natl.Acad.Sci.USA93(1996)Table1.PNDsdefinedbyautoimmuneantibodiesNeurologicsyndromeParaneoplasticnamePhenotypeTumorAntibodyCerebellardegenerationPan-cerebellardysfunction(Purkinjecell)TypicalBrea 868CellBiology:OsbornandWeberFIGS.1-7.(Legendappearsatbottomofthefollowingpage.)Proc.Nat.Acad.Sci.USA73(1976) Proc.Nat.Acad.Sci.USA73(1976)869FIG.8.Recoveryofcytoplasmicmicrotubulesin3T3cellsafterColc Proc.Natl.Acad.Sci.USA75(1978)RESULTSWhennormalhumanglobinDNAwasdigestedwithEcoRIandprobedwithamixtureofa-and(3-globincDNAs,fivemajorfragmentswereseen(Fig.1).Thelargestfragment,about21kilobases(kb)inl Proc.Natl.Acad.Sci.USA92(1995)FIG.1.CytologyofDrosophilafemalemeiosis.Theimagesareconfocalmicrographsofoocytesfixedandlabeledwithantibodiestovisualizemeioticstages.(A)MetaphaseIarrestinamaturestage14o Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 TheNunproteinofbacteriophageHK022isamemberofthearginine-richmotiffamilyofRNAbindingproteins,whichincludesthephageNtranscriptionantiterminationproteinandtheHIVTatandRevproteins(Fig.1;refs.1±3).Nunbind Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby 2612Biochemistry:Sancaretal.PstIHaelilt*.JHinfl-TaqIadzJHpa11ti.r4HhaI'recAmRNA-recAprotein-Proc.Nati.Acad.Sci.USA77(1980)EcoRI-9A.-ii4------200020040060080010001200BasepairsFIG.1.Restrictionmapandseq

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