MyStem ® Autograft Preparation Kit - PowerPoint Presentation

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MyStem®Autograft Preparation Kit

Regenerative Treatments made easy

The People

Pier Ivona,

IFATS Member, WOSIAM Member,

Regenerative Medicine Expert, Entrepreneur

Mr. Ivona has a multi year experience in Regenerative treatments and MedDev industry,

H

is experience leads the team who developed MyStem technology

The Science - Stromal tissue -

Scientific Definition:

The connective tissue framework of 

anorgan

, a gland, or 

other structure

, as distinguished from the 

tissues

 performing the 

special function

 of the organ or part.

Stroma

is a broadly used term for the loose connective tissue that contains mesenchymal stem cells and other cells like fibroblasts, macrophages, adipocytes, mast cells and

leukocytes.

Source:

The

American Heritage® Stedman's Medical

Dictionary

The Science - Stromal tissue –Why Adipose stromal tissue a Regenerative fraction source?

Adipose tissue as a Stromal Tissue source provides regenerative components in high ratio

The Science - Stromal tissue –

Adipose tissue as a Stromal Tissue source provides regenerative components in great numbers (Stromal Vascular Fraction-SVF)

The Stromal Vascular Fraction (SVF)

Derived from Blood Vessels in adipose (fat) stromal tissueCapillaries are primary source (see inset below) Same-day clinical procedureEasy to harvestHigh cell counts

Blood vessels / Capillaries

Adipose (fat) cells

The Stromal Vascular Fraction (SVF)

The isolated SVF contains

:

Adult Stem CellsEndothelial CellsFibroblast CellsPericytesRed Blood CellsWhite Blood Cells … and much more

ADSCs are Growth Factors and Cytokines factories

Growth Factors epidermal growth factor (EGF) vascular endothelial growth factor (VEGF) basic fibroblast growth factor (bFGF) keratinocyte growth factor (KGF) platelet-derived growth factor (PDGF), hepatocyte growth factor (HGF) transforming growth factor-beta (TGF-β) insulin-like growth factor (IGF) brain-derived neurotrophic factor( BDNF)

Cytokines

Flt-3 ligand,

granulocyte colony stimulating factor (G-CSF)

granulocyte/macrophage colony stimulating factor (GM-CSF)

macrophage colony stimulating factor (M-CSF)

interleukin-6 (IL-6)

interleukin-7 (IL-7)

interleukin-8 (IL-8)

interleukin-11 (IL-11)

 interleukin-12 (IL-12)

leukemia inhibitory factor (LIF)

tumor necrosis factor-alpha (TNF-α)

The Technology - the MyStem concept -

Our Vision: Providing Regenerative fraction at the point-of-care

MYSTEM® Process - Step by Step –(can be repeated several times for the same patient)

MYSTEM® SVF isolation Process(the process can be repeated several times for the same patient)

1.Tissue Harvesting

2.Fractioning

3.Cells Separation

4.Cells Concentration

Fat Fraction

Liquid Fraction

7.1

millions cells / harvested

ml

up

to 10x volume

reduction

The Technology - the MyStem device -

Single-use Medical DeviceOne-step ProcedureProcess time 15 min710.000 Nucleated Cells / mlUp to 10x ConcentrationInternational Patent

Clinical use of MyStem technology

Demonstrated effects in:

Bone tissue repair (Published Study)

Osteoarthritis (Case Report)

Intervertebral Disc disease (Published Study)

Tendon injury (Case Report)

Severe Burns (Case Report)

Soft tissue augmentation (Case Report)

Wound healing (Case Report)

Diabetic Foot Syndrome (Case Report)

Crohns (Case Report)

Fistulas (Case Report)

MyStem Factsheet

MyStem Authorizations CE MarkSFDA (Saudi FDA)TGA (Australia)ANVISA (Brazil)Clinical Evidenceover 1200 clinical cases done

...helping people...

Together.....

..with great results..

Thank you