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Backup not only for computers!
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Slide1

Backup not only for computers!

Berger B. Knapp P. Gallnböck M.

© SZV OÖ

© SZV OÖ

27

th

Annual Meeting

of

DAGENE, Germany

Slide2

Introduction

Frozen boar semen – since

> 40 yearsThalheim > 25 years Special uses – not for routine AIScientificExportTransport Planned

mating

Genebank – e.g. backup for

cattle AI since 1997Mangalica, Turopolje

in Austria since 2000Slide3

Frozen boar

semenAbout 30% of boars

not freezableNon-return rates not really satisfying Smaller litter size Huge

amount of

sperm cellsLess

if deep intra-uterine inseminationHandling

of semen

more complicated Processing and

freezing Thawing and re

-dilution…but long-time storage is

possible!Slide4

Why another

cryo-project?Biggest Austrian pig

AI-centre 8 km from Genebank 270 boars, 5 breeds, PRRS-free statusOwned

by pig

breeding associationPRRS outbreak

2012, all boars slaughteredHuge

financial loss

to centre and

producersRe-stocked boars not first

qualityLoss of breeding progress

Safeguard against loss of Top-Genetics neededSlide5

The project

Cooperation between Pig

Breeders Association and GenebankScreen all TOP-Genetics boars for freezability

of semen

Conserve freezable boars

in GenebankDo not disrupt

semen

collection routine at AI centre!Slide6

Material & Methods

1Screening: 62 ejaculates, 28 TOP-

boars (15 Piétrain, 7 Landrace, 4 Large White, 1 Duroc, 1 Schwäbisch-Hällisch) Collection within routine of

AI centre,

20ml of sperm-rich fraction

of ejaculateDilution 1:1 with BTS

extender (DiluPorc

TM)Fresh

semen analysis CASA (SpermVision,

minitube): Motility (mot

), DensityTransport to GenebankAcrosome integrity (acr

) – smears stained acc. KOVACS & FOOTE (1992)Slide7

Material & Methods 2

Cryo-conservation at Genebank acc

. WESTENDORF et al. (1975) mod. EGERSZEGI et al. ( 2009)Extender Androstar Cryo Plus (minitube)0,5 ml medium French straws,

concentration 1x109

cells/mlThawing at AI centre

37°C for 20 sec.Re-dilution with BTS-extender

, CASA, smears

Minimal requirements: 30% intact acr, 30%

progressive motConservation: 5 to 9 double AI

doses/boarOne whole

ejaculate neededSlide8

Results – Screening

fresh

thawed

62

ejaculates

28

boars

70%

blocks

Minimum/Maximum

Median

acr

mot

30%

ejaculates

discarded

!

3

boars

not

freezable

!

Slide9

Results – Genebanking

fresh

thawed

25

ejaculates

25

boars

0

ejaculates

discarded

!

All

boars

freezable

!

Slide10

Discussion

Success rate in line

with literatureStill 30% negative ejaculates in screeningStill 13 boars at least 1 negative resultFreezability of

semen Individual

trait – genetic determination?Fresh

semen quality allows

no predictions!

Sexual resting time important (< 5 days

)5 x 109 sperm cells per dose!

Much semen needed for

Cryo (one whole ejaculate)Intra-uterine insemination not used by breeders

rfSlide11

Conclusions

Enhanced security for breeders and

breeders associationScreening well integrated into routine Backup collection owned

by breeders

associationstored at Genebank (extra tank)

for 5 yearsAfter 5 years 2 double

doses into

core collection – owned by

state, rest destroyedExport

orders pendingSlide12

Thank

you

for listening!11 piglets

*

10.09.2014

from semen

frozen

17.07.1993!

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Backup not only for computers! - Description


Berger B Knapp P Gallnböck M SZV OÖ SZV OÖ 27 th Annual Meeting of DAGENE Germany Introduction Frozen boar semen since gt 40 years Thalheim gt 25 ID: 611488 Download Presentation

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