PPT-Fluorescence Spectroscopy

1 Dr Nikhat Siddiqi Principles Interaction of photons with molecules results in promotion of valence electrons from ground state orbitals to high energy levels The molecules are said to be in excited state. 5. . Discussion, Conclusions, and . Future Steps. Choosing Martian Samples for Fossil . Biosignature. Analysis of . Kerogen. : . . Fluorescence . microscopy and Raman spectroscopy applications to analog samples. . M. . Uherek. 1. , J. . Fischerova. 2. . and. . D. . Chorvat. . jr. .. 1,2. 1. International Laser Centre, Bratislava, Slovakia . 2. Faculty of Mathematics, Physics and Informatics, Comenius University,. . a . semi-analytical ocean color model for MODIS and beyond. Stéphane. . Maritorena. & Dave Siegel. Earth Research Institute. University of California, Santa Barbara. - . Develop. a . fluorescence . Hitachi-. HiTec. Electronic Excitation Model. A. ~. X. ~. g. Excitation. (. Absorption. ). Radiationless. decay. (vibrational relaxation). g. Fluorescence. . (Fast . radiative. decay). Internal conversion. Techniques for Archaeological Analysis. LIBS. Raman. LIF. Laser Induced Breakdown Spectroscopy (LIBS). LIBS set-up. LIBS. Advantages. LIBS in . Pigment . Identification. Chemical Identification . Pigment Identification. Daniel . Ren. čiuk. IBP AS CR. S1001 / 2016 - CEITEC. Contents. Brief background. Absorption spectroscopy (AS). Electronic (UV/Vis). Vibrational. (IR). Raman scattering. Emission spectroscopy. Fluorescence. UV Spectroscopy and Qualitative Analysis. UV-vis spectroscopy is usually not very useful for qualitative analysis because there are few absorption maxima and minima. Solvents:. Must be transparent in region of interest. Chemistry . 243. Luminescence. Emission of photons accompanying the relaxation from an excited to a ground state.. Photoluminescence—Excited state generated by absorption of a photon.. Fluorescence and phosphorescence. Chlorophyll Concentrations . of Vegetables. Jane Kim & Rebecca Long. Ch251B – Instrumental Analysis Lab. Professor A. . Newmark. Background – Why Chlorophyll?. Health benefits in chlorophyll:. Mark . Schnittker . 2013, Aug-23. schnittker@yahoo.com. 408-368-1064. . Some common measurement techniques. . . Atomic techniques (usually inorganic). X-ray spectroscopy. Optical absorption. Plasma discharge. g. n. i. n. O. H. O. M. e. O. H. O. l. i. g. n. i. n. M. e. O. O. l. i. g. n. i. n. O. H. O. M. e. O. H. H. O. . O. O. M. e. Dissolved. Organic . Matter. Slides and figures from Mark Williams, Diane McKnight, Bailey Simone, Rose Cory, Matt Miller, Rachel Gabor, . 2. Nuclear Magnetic Resonance Spectroscopy. After hydrogen, the most useful atom providing information is carbon-13.. The . overall intensity of a . 13. C signal is about 6400 . times . less than the intensity of an . Dr. . . (. Mrs. ). Uma. . Sharma. Professor. School of Studies in Chemistry & Biochemistry, . Vikram. University,. UJJAIN (M.P.) . S.S. IN CHEMISTRY & BIOCHEMISTRY. Based on the properties of X-rays, a number of . It is a phenomenon of emission of radiation when the molecules are exited by radiation at certain wavelength.. FLUORIMETRY:-. It is measurement of fluorescence intensity at a particular wavelength wit the help of a filter fluorimeter or a spectrofluorimeter..