PPT-NATL-east

Author : natalia-silvester | Published Date : 2017-10-26

No sites in NATLeast See larger map for details NATLwest Definitions Legacy site Site found and declared cleared prior to

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NATL-east: Transcript

No sites in NATLeast See larger map for details NATLwest Definitions Legacy site Site found and declared cleared prior to . Proc.Natl.Acad.Sci.USA93(1996)Table1.PNDsdefinedbyautoimmuneantibodiesNeurologicsyndromeParaneoplasticnamePhenotypeTumorAntibodyCerebellardegenerationPan-cerebellardysfunction(Purkinjecell)TypicalBrea Proc.Natl.Acad.Sci.USA93(1996)11529BothLandL*canbeprotonatedonLys-156,butthepKaforprotonationisconsiderablylowerforL*thanforL;pKL*iswhilepKLis10.OneimportanttenetofthismodelisthattheIndsmutationsincre Proc.Natl.Acad.Sci.USA90(1993)2031dues,0.98forLexA-C52containingasinglecysteine,and0againforLexA-C52aftermodificationwith4-azidophenacylbromide,suggestingthatessentiallyallthecysteinesidechainshaverea 6148Biochemistry:LittleandHarperTable1.BacterialstrainsemployedSource,reference,StrainRelevantgenotypeorderivationAB1157recA+lexA+sup-37(15)DM49recA+lexA3sup-37(12)DM511recA+IexA3ts1-1sup-37(16)DM1187 I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco Proc.Natl.Acad.Sci.USA90(1993)pHP45QC(13)bySmaIdigestionandgelpurification.pIB415isaderivativeofpIB410(ofIncPreplicon)containingvirAA63/240,avirAderivativelacking178aminoacidsintheperiplasmicdomain(14 Genetics:Slupskaetal.Table1.ComparisonofmutAandmutCwithmutA,CStrainLac+revertantsper(CC105)108cells,no.+2.3 Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby Proc.Natl.Acad.Sci.USA93(1996)2583multiplexamplificationasdescribedabove.Theonlydiffer-encebetweenthesetwoproceduresisthatsomePprimersusedinMGSmaycontainsingle-basemismatchestothetemplatestoconvertthe Proc.Natl.Acad.Sci.USA82(1985)6415fragmentwasisolatedfroma0.8%agarosegelbyusingtheapparatusforpreparativegelelectrophoresis.DNASequencing.DNAsequencingusingdideoxynucleo-tideswasperformedasdescribedby 4800Biochemistry:Birchmeieretal.Proc.Natl.Acad.Sci.USA87(1990)1CCGCATTCAAGCTTTCAAGCATTCAMGGTCTAAATGAAAAAGGCTAAGTATTATTTCAAAAGGCAAGTAT7172ATCCTAAT~TAGCAAACAAAGCAAATCCATCAiCTACTCCTCcMTTGAGTGATGAGCCCATAT ProcNatlAcadSciUSA8419878231tractionwithisopropylalcoholsaturatedwith30MNaCl/03MNa3citratepH70Theaqueousphasecon-tainingtheDNAwasdialyzedagainst10mMTris1HClpH76/1mMNa2EDTAScreeningofaAgtl1GenomicLibra ProcNatlAcadSciUSA821985tiononthelocationorientationandcopynumber26IntheexperimentsrepresentedinFig4theoligonucleotideTATATAATAwasinsertedintotheXhoIsiteofplasmidspFN7x-101-170-210-407and-99seeFig1Tho 564MedicalSciencesSawyersetaltientsweretreatedattheUniversityofCaliforniaLosAngelesandwereconditionedwithintravenouscyclophos-phamideat60mg/kgfor2daysandeithertotalbodyirradiationwith1125cGygiveninfiv

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