15875 Gaither DriveGaithersburg MD 20877FAX 3015604919TEL 3013305966Toll Free 18669186812Email infosignagencomWeb wwwsignagencomIntroductionGenJetIn Vitro DNA TranfectionReagent Ver II is upgra ID: 880444
Download Pdf The PPT/PDF document "Cat SL100489SIHAStoreat In Vitro DNA Tr..." is the property of its rightful owner. Permission is granted to download and print the materials on this web site for personal, non-commercial use only, and to display it on your personal computer provided you do not modify the materials and that you retain all copyright notices contained in the materials. By downloading content from our website, you accept the terms of this agreement.
1 Cat # SL100489-SIHAStoreat In Vitro DNA
Cat # SL100489-SIHAStoreat In Vitro DNA TransfectionReagent for SiHaCells (Ver. II)A Protocol for Transfectionsof SiHaCells 15875 Gaither DriveGaithersburg, MD 20877FAX. 301-560-4919TEL. 301-330-5966Toll Free. 1-(866)-918-6812Email: info@signagen.com Web: www.signagen.com Introduction:GenJetIn Vitro DNA TranfectionReagent (Ver. II) is upgraded Cells should be plated 18 to 24 hours prior to transfectionso that the monolayer cell density reaches to the optimal ~80% confluencyat the time of transfection. Complete culture medium with serum and anti-bioticsis freshly added to each well ~60 minutes before transfection. This product is for laboratory research ONLY and not for diagnostic use2008 SignaGen LaboratoriesStep B. Preparation of GenJet-DNA Complex and TransfectionProceduresFor SiHacells, the optimal ratio of GenJetL):DNA (g) is 3:1. To ensure the optimal size of complex particles, we recommend using serum-free DMEM with High Glucose to dilute DNA and GenJetReagent. The following protocol is given for transfectionin 24-well plates, refer to Table 2 1.2 2.4 x 1096-well Plate4.0 8.0 x 1048-well Plate0.8 1.6 x 1024-well Plate1.5 3.0 x 1012-well Plate4.0 8.0 x 106-well Plate3.5 7.0 x 1035 mm Dish0.9 1.8 x 10 2 x 0.0196-well2 x 0.0524-well 2 x 0.0248-well 150 -3002 x 1.2550 -100250 ml flask54 -1082 x 0.7518 -36T75 flask21 -242 x 0.57 -810 cm dish 2 x 0.25 Table 2. Recommended Amounts for Different Culture Vessel Formats