of Mangosteen Inhibits Nuclear Factor κ B NF κ B Activation and Reduces ICAM1 and IL6 in Atherosclerosis Department of Cardiology Brawijaya Medical School Saiful ID: 318140
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Slide1
Pericarp
of
Mangosteen Inhibits Nuclear Factor κB (NF-κB) Activation and Reduces ICAM-1’ and IL-6 in Atherosclerosis
Department of Cardiology, Brawijaya Medical SchoolSaiful Anwar General Hospital
Djanggan Sargowo1,2, Hendarto1, M. Saifur Rohman2
1Slide2
Background
Atherosclerosis is a major cause of mortality and morbidity not only in the developed countries but also in developing countries
Atherosclerosis is widely viewed as an inflammatory disease with hypercholesterolemia being a dominant underlying risk factor2Slide3
Karatzis
EN.
Hell J Cardiol 2005; 46: 232-239The Role of Inflammatory Agents in Endothelial Function and their Contribution to Atherosclerosis3Slide4
Model for the role of
NF-
κB/Rel transcription factors in atherogenesisBrand K, Page S, et al. Experimental Physiology 1997; 82: 297-304Pericarp of mangosteen?4Slide5
Objective
To examine whether
mangosteen’s pericarp inhibit NF- κB activation, IL-6 and ICAM-1 expression in high cholesterol diet rats5Slide6
6
Simple Random sampling
Rattus novergicus wistar strain divided into 5 treatment groupsGroup INormal dietGroup II
Group IIIGroup
IVGroup V
Blood sample and aortic arch tissue were examined at week 12
th
I
mmunohistochemistry
staining
of
NF-
κ
B
& ICAM-1 or
IL-6 protein by ELISA methods
To examine
NF-
κ
B activation,
ICAM-1 expression and IL-6 level
Data analysis
Mangosteen peel extract
200mg/kg (po
)
Mangosteen peel extract
400 mg/kg (po
)
Mangosteen peel extract 800
mg/kg (po)
High cholesterol diet
MethodsSlide7
RESULTS
7Slide8
NF
-
κB ActivationNormal diet (Negative Control)High cholesterol diet (Positive Control)the arrows indicate brown discoloration in the cell nucleusthe arrows indicate brown discoloration in the cell nucleus
p = 0.000 Immunohistochemistry staining, magnification 200x
8Slide9
High cholesterol diet +
Mangosteen’s
pericarp Extract 200 mg/kgNF-κB ActivationHigh cholesterol diet +
Mangosteen’s pericarp Extract 400 mg/kgHigh cholesterol diet + Mangosteen’s pericarp Extract 800 mg/kgthe arrows indicate brown discoloration in the cell nucleus
the arrows indicate brown discoloration in the cell nucleusthe arrows indicate brown discoloration in the cell nucleusImmunohistochemistry staining, magnification 200x
9Slide10
NF-
к
B ActivationOne way ANOVA, Post Hoc analysis10Slide11
High cholesterol diet (Positive Control)
Normal diet (Negative Control)
ICAM-1 Expressionarrows show brown discoloration in the cytoplasmarrows show brown discoloration in the cytoplasm
p = 0.000 Immunohistochemistry staining, magnification 200x11Slide12
ICAM-1 Expression
High cholesterol diet +
Mangosteen’s pericarp Extract 200 mg/kgHigh cholesterol diet + Mangosteen’s
pericarp Extract 400 mg/kgHigh cholesterol diet + Mangosteen’s pericarp Extract 800 mg/kgarrows show brown discoloration in the cytoplasmarrows show brown discoloration in the cytoplasmarrows show brown discoloration in the cytoplasm
Immunohistochemistry staining, magnification 200x12Slide13
ICAM-1 Expression
One way ANOVA, Post Hoc analysis
13Slide14
Il-6 Protein Level
Data Transformation, One way ANOVA, Post Hoc analysis
14Slide15
DISCUSSION
γ
- Mangostin inhibited COX-1 and -2 activity and PGE-2 synthesis in C6 rat glioma cells, inhibited LPS-induced expression of COX-2 protein and its mRNA, reduced the LPS-inducible activation of NF-кB1Mangostin is acting as a free radical scavenger to protect the LDL from oxidative damage in vitro system2 Nakatani, et al Mol Pharmacol. 2004 Sep;66(3):667-74Williams P, et al Free Radic Res. 1995 Aug; 23(2): 175-8415Slide16
Xanthones significantly
inhibit TNF-
α-induced increase of ICAM-1 expression in cultured endothelial cells 1Mangostin showed protective effect against isoproterenol-induced oxidative damage and myocardial injury in rats2α-Mangostin, i.p. has anti-inflammatory effects in several experimental models of inflammation in rats and guinea pigs3 Madan B et al Bioorg Med Chem 2002;10:3431–3436Devi Sampath and Vijayaraghavan 2007Gopalakrishnan et al.198016Slide17
CONCLUSION
High cholesterol diet group resulted in significantly increased NF-
кB activation, ICAM-1 expression400 and 800 mg/kg pericarp extract inhibited NF-кB activation and reduced ICAM-1 protein expression in high cholesterol ratsThe extract of mangosteen peel significantly inhibits NF-κB activation and reduces ICAM-1 and IL-6 expression in high cholesterol rats17Slide18
18Slide19
19Slide20
20
Thank You