PRODUCTION AND APPLICATION OF AMYLASE - PowerPoint Presentation

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Uploaded On 2022-02-14

PRODUCTION AND APPLICATION OF AMYLASE - PPT Presentation

GROUP 5 SUBMITTED TO Dr Hajra Sadia GROUP MEMBERS Maarej Khan Zara Taimoor Rameeza Hassan Maryam Jadoon Hanzala Jahangir Maham Nadeem Shehrbano Akhtar ID: 908716

fermentation amylase medium production amylase fermentation production medium industry starch enzymes organisms bacteria group

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Slide1

PRODUCTION AND APPLICATION OF AMYLASE

GROUP # 5

SUBMITTED TO :

Dr.

Hajra

Sadia

GROUP MEMBERS :-

Maarej

Khan

Zara

Taimoor

Rameeza

Hassan

Maryam

Jadoon

Hanzala

Jahangir

Maham

Nadeem

Shehrbano

Akhtar

Slide2

AMYLASE :

enzymes that break down starch or glycogen

produced by a variety of living organisms: bacteria, plants and humans.

major advantage of using micro organisms for the production of amylases is in economical bulk production capacity and microbes are also easy to manipulate to obtain enzymes of desired characteristics

α-amylase (alpha-amylase) - Reduces the viscosity of starch by breaking down the bonds at random, therefore producing varied sized chains of glucose

Slide3

Microbial production of amylase

MICROORGANISM:

Bacillus spp. Why bacteria? Why not fungus?

INOCULUM AND FERMENTATION MEDIUM:

addition of sterile distilled water into nutrient agar slants, and incubated.

FERMENTATION MEDIUM:-Bacteriological peptone -Magnesium sulfate-Potassium chlorate -StarchEXTRACTION OF AMYLASE FROM FERMENTATION MEDIUM: fermentation medium is harvested by centrifugation at 5000 rpm for 20 min at 4°C.The supernatant is collected to estimate the amylase activitySUBMERGED FERMENTATION: why advantageous?OPTIMUM TEMPERATURE: 35°COPTIMUM pH: 7OPTIMUM TIME: 10hours

Slide4

“APPLICATIONS”

Starch Conversion

Detergents Industry

Fuel Alcohol Production

Food and Beverages for example in sweets, syrups, baked goods, ice-cream or tomato ketchup Textile Industry Paper Industry

Slide5

Thank you!