Yeast extract - PDF document

Lactose Gelatin Medium, Modified is recommended for detection and presumptive identification of Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 Suspend 16 grams in 100 ml warm distilled water. Heat to boiling to dissolve the medium completely and dispense 10 mlamounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just beforeMembers of the genus Clostridium Reaction±0.1 pH 7.0-7.0 Cultural ResponseM987: Cultural characteristics observed under anaerobic conditions, after an incubation at 35-37°C for 24-48 hours. HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens 50-100 luxuriant acid and gas positive Clostridium paraperfringens 50-100 good acid production positive 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : / 201 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not Please refer disclaimer Overleaf.Lactose Gelatin Medium, ModifiedM987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 Suspend 16 grams in 100 ml warm distilled water. Heat to boiling to dissolve the medium completely and dispense 10 mlamounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just beforeMembers of the genus Clostridiumare distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are: Clostridium, which produces one of the most potent toxins in existence; Clostridium tetani , causative agent of tetanus; Clostridium perfringens , commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Clostridium . Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detectionand yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite Reaction±0.1 pH 7.0-7.0 Cultural Response Please refer disclaimer Overleaf.Lactose Gelatin Medium, ModifiedM987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 Suspend 16 grams in 100 ml warm distilled water. Heat to boiling to dissolve the medium completely and dispense 10 mlamounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just beforeMembers of the genus Clostridium are distributed widely in nature and are found in soil as well as in fresh water and marinesediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are: Clostridium , which produces one of the most potent toxins in existence; Clostridium tetani , causative agent of tetanus; Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Clostridium . Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detectionTryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite Reaction±0.1 pH 7.0-7.0 Cultural Response Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 Suspend 16 grams in 100 ml warm distilled water. Heat to boiling to dissolve the medium completely and dispense 10 mlamounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just beforeMembers of the genus Clostridium are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are: Clostridium , which produces one of the most potent toxins in existence; Clostridium tetani , causative agent of tetanus; Clostridium perfringens , commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Clostridium . Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detectionTryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite Reaction±0.1 pH 7.0-7.0 Cultural Response Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 Suspend 16 grams in 100 ml warm distilled water. Heat to boiling to dissolve the medium completely and dispense 10 mlamounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just beforeMembers of the genus Clostridium are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are: Clostridium , which produces one of the most potent toxins in existence; Clostridium tetani , causative agent of tetanus; Clostridium perfringens , commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Clostridium . Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detectionTryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite Reaction±0.1 pH 7.0-7.0 Cultural Response Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 Suspend 16 grams in 100 ml warm distilled water. Heat to boiling to dissolve the medium completely and dispense 10 mlamounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just beforeMembers of the genus Clostridium are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are: Clostridium , which produces one of the most potent toxins in existence; Clostridium , causative agent of tetanus; Clostridium perfringens , commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Clostridium . Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detectionTryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite Reaction±0.1 pH 7.0-7.0 Cultural Response Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 Suspend 16 grams in 100 ml warm distilled water. Heat to boiling to dissolve the medium completely and dispense 10 mlamounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just beforeMembers of the genus Clostridium are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are: Clostridium , which produces one of the most potent toxins in existence; Clostridium tetani, causative agent of tetanus; Clostridium perfringens , commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Clostridium . Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detectionTryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite Reaction±0.1 pH 7.0-7.0 Cultural Response enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 **Formula adjusted, standardized to suit performance parameters Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Members of the genus Clostridium are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are: Clostridium , which produces one of the most potent toxins in existence; Clostridium tetani , causative agent of tetanus; Clostridium perfringens , commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Clostridium . Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detectionTryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite Reaction±0.1 pH 7.0-7.0 Cultural Response enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringensATCC 12924 50-100 luxuriant acid and gas positive Clostridium paraperfringensATCC 27639 50-100 good acid production positive Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : / 201 must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 **Formula adjusted, standardized to suit performance parameters Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens 50-100 luxuriant acid and gas positive Clostridium paraperfringens 50-100 good acid production positive Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : / 201 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 **Formula adjusted, standardized to suit performance parameters Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Tryptose 15.000 Yeast extract 10.000 Lactose 10.000 Disodium phosphate 5.000 Gelatin 120.000 Phenol red 0.050 Final pH ( at 25°C) 7.±0.1 **Formula adjusted, standardized to suit performance parameters Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : 02 / 2016 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notReaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg lbx ad qdedqqdc hm hmchuhctbk HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : 02 / 2016 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notReaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg lbx ad qdedqqdc hm hmchuhctbk HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : 02 / 2016 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notReaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd lbx ad qdedqqdc hm hmchuhctbk HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : 02 / 2016 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notReaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd lbx ad qdedqqdc hm hmchuhctbk Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : 02 / 2016 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notReaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : 02 / 2016 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringensATCC 12924 Clostridium paraperfringensATCC 27639 Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : 02 / 2016 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notReaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reference 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Revision : 02 / 2016 Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd lbx ad qdedqqdc hm hmchuhctbk User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the ClostridiumDownes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,Revision : 02 / 2016 Technical Data HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories Please refer disclaimer Overleaf. User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,Revision : 02 / 2016 Technical Data HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd oqnctbs lbx Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd lbx ad qdedqqdc hm hmchuhctbk User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:1.Murray R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,Revision : 02 / 2016 Technical Data HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd ad qdedqqdc hm hmchuhctbk User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the ClostridiumDownes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,Revision : 02 / 2016 Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd qdedqqdc hm hmchuhctbk Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd oqnctbs Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed.,Revision : 02 / 2016 Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd lbx ad hm hmchuhctbk Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., 02 / 2016 User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories Pgac C,U,* 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Revision : 02 / 2016 Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (1). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in accordance with AOAC (3) and a slight modification of this medium is recommended by APHA for detection Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd lbx ad qdedqqdc User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,2.Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Clostridium3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Revision : 02 / 2016 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC.4.Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Revision : 02 / 2016 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Revision : 02 / 2016 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology,3.FDA Bacteriological Analytical Manual, 2005, 18th Ed., AOAC, Washington, DC. Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Revision : 02 / 2016 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Revision : 02 / 2016 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories 1.Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Revision : 02 / 2016 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Revision : 02 / 2016 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories Revision : 0 Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Reference 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories Revision : 0 Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Reference 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Reference 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in Clostridium perfringens Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd lbx ad qdedqqdc hm User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories Revision : 0 Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Reference 1. Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 amounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and marinewater sediments throughout the world (). Clostridial species are one of the major causes of food poisoning / gastro-intestinalillnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of tetanus;Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to damagethe host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, Modifiedis prepared in Clostridium perfringens Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol redacts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from Tryptose Sulphite enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd lbx ad qdedqqdc hm hmchuhctbk User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notHiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, Customer care No.: 022-6116 office : A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email: Technical Data HiMedia Laboratories Revision : 0 Technical Data Organism Inoculum(CFU) Growth Lactose Gelatin Clostridium perfringens Clostridium paraperfringens Reaction of 16.0% w/v aqueous solution at 25°C. pH : 7.5±0.1 HiMedia Laboratories sgd gxfqnrbnohb sgd oqnctbs lbx Trd adenqd dwohqx cbsd nm sgd kbadk- User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with sample must Reference 1.Baird R.B., Eaton A.D., and Rice E.W., (Eds.), 2015, Standard Methods for the Examination of Water and Czeczulin J. R., Hanna P. C., Mcclane B. A., 1993, Cloning, nucleotide sequencing, and expression of the Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., Murray P. R., Baron J. H., Pfaller M. A., Jorgensen J. H. and Yolken R. H., (Ed.), 2003, Manual of Clinical Microbiology, Please refer disclaimer Overleaf. M987 Composition** Ingredients Gms / Litre Directions Suspend 16 grams in 100 ml warm otqhehdc . distilled water. Heat to boiling to dissolve the medium completely and dispense 10 mlamounts in 15x150 mm screw capped tubes. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Just Principle And Interpretation Members of the genus are distributed widely in nature and are found in soil as well as in fresh water and water sediments throughout the world (). Clostridial species are one of the major causes of food poisoning / gastro-illnesses. They are gram-positive, spore-forming rods that occur naturally in soil (2). Among the family are:, which produces one of the most potent toxins in existence;Clostridium tetani, causative agent of Clostridium perfringens, commonly found in wound infections and diarrhoea cases. The use of toxins to the host is a method deployed by many bacterial pathogens including Lactose Gelatin Medium, is prepared in accordance with AOAC () and a slight modification of this medium is recommended by APHA for Tryptose and yeast extract in the medium provide essential growth nutrients. Lactose is the fermentable sugar and phenol acts as fermentation indicator, which changes from red to yellow due to acid production. Following incubation the medium tubeis chilled for 1 hour at 5°C, if medium gels; it should be incubated for an additional 24 hours to examine gelatin liquefaction.The medium is stab inoculated with pure Fluid Thioglycollate Medium (M009) culture or isolates from enq cdsdbshnm bmc oqdrtloshud hcdmshehbbshnm ne Bknrsqhchtl odqeqhmfdmr eqnl ennc hm bbbnqcbmbd vhsg Qdbc sgd lbx ad qdedqqdc hm hmchuhctbk