PPT-Functional organization of the yeast proteome by systematic

Presented by Nathalie Kirshman and Xinyi Ma Background Protein complexes TAP tandemaffinity purification Background protein complexes At the biochemical level proteins rarely act alone rather they interact with others to perform cellular tasks. 2012 HHMI Summer Workshop for High School Science . Teachers. The Genomics of . S.cerevisiae. GOALS. Introduction to the Genomics of Yeast. Sequencing Technologies and how they are evolving. Introduction to Systems Biology and modern Yeast Genetics. Michelle . Attner. July 24, 2012. What is budding yeast, . S. . cerevisiae. ?. Electron micrograph. DIC (light microscopy). Advantages to budding yeast as a model organism. Simple, eukaryotic cell (~10μm diameter). a graph . database backed protein analysis software. Thilo Muth. MPI Magdeburg / Germany. (cooperation with CompOmics group in Ghent / Belgium). Life Sciences + Graph Database?. Life scientists are interested in genes, proteins, metabolites, relationships, interactions and biological networks.... Breads. Yeast Breads in General. They have a distinctively appealing sweet smell and delicious taste that cannot be matched to commercially prepared sandwich breads.. Many meal managers rely on the ease of bread machines to make homemade bread an option to their menu plans.. Y2.U8.2. Types of Dough. Lean Dough. Flour, water, yeast, salt. Chewy texture, crisp crust. French bread, hard rolls. Rich Dough. Contain tenderizing ingredients. Shortening, butter, sugar milk. Parker House rolls, brioche, cinnamon bun, Danish. SDS-PAGE gives a snapshot of proteins in an extract. Proteins are extracted from cells. There are major challenges in analyzing yeast cell proteins!. How can proteins be efficiently extracted without being degraded? . , . Miraflores. de la Sierra, 10-11 December 2012. HPP. Sp-HPP. Use of SEQUEST search results with ProteoRed.org MIAPE Extractor. A working Workflow to extract MIAPE information from Proteome Discoverer 1.3 search results using . Objective. Explain how dough for yeast-raised products is prepared.. Dough Preparation. Preparing dough for a yeast-raised bakery product is not as easy as preparing . doughs. and batters for quick breads. YSC1021 Yeast: Yeast Knock Out StrainYSC4300Yeast Heterozygous (plates 281-282)YSC1053Yeast MATa CollectionYSC4343Yeast Heterozygous Plate 282YSC4298Yeast MATa (plates 72-73)YSC4508Yeast Heterozygous GLUTEN – provides dough with . elasticity. and . strength. .. YEAST – makes dough . rise. and gives it it’s porous . texture. Under developed gluten. Developed gluten. Define. HYDRATION. To Add water. B. ottled Coopers Ale . Yeast. Kieren Vercoe. The Premise…. Reactivated yeast from 6 stubbies will comfortably ferment out a 1.063 OG WORT (assuming a 20 L batch). Reactivated . Yeast. …?. Bringing it out of dormancy…. Joshua Egan. 10. th. . grade, Pittsburgh Central Catholic High School, 2. nd. year in PJAS . Problem. Cologne is used worldwide with unknown effects on skin microflora. Microbial Flora . Little is known about the association between humans and their flora. Steven Chang, . Senior Research Advisor (Library – Science, Health & Engineering). La Trobe University. Patrick Condron. Research Librarian (Medicine, Dentistry and Psychology). University of Melbourne. Volume 1, Issue 1 Culinary Arts II A WHAT IS IT? THERE ARE SEVERAL TR ADITIONAL METHODS FOR CREAT- ING STARTERS FOR BREADS. ALL OF THESE STARTERS ARE EASY TO PREPARE. NSISTS OF A SIMPLE MIXTURE OF W