PPT-Preparing yeast cell extracts

SDSPAGE gives a snapshot of proteins in an extract Proteins are extracted from cells There are major challenges in analyzing yeast cell proteins How can proteins be efficiently extracted without being degraded . 2012 HHMI Summer Workshop for High School Science . Teachers. The Genomics of . S.cerevisiae. GOALS. Introduction to the Genomics of Yeast. Sequencing Technologies and how they are evolving. Introduction to Systems Biology and modern Yeast Genetics. Cool-Rise . Doughs. Designed to rise slowly in the refrigerator. . Mix ingredients and knead dough. Brief rest, shape dough. Place in a pan . Cover dough and place in refrigerator. Ready (risen) in 2 to 24 hrs. . Objective. Explain how dough for yeast-raised products is prepared.. Dough Preparation. Preparing dough for a yeast-raised bakery product is not as easy as preparing . doughs. and batters for quick breads. Microbiologists have developed techniques for isolating and maintaining strains in the laboratory. Strains: microorganisms of the same species that are descended from a single cell and possess the same genotype. Yeast . yeast (. Saccharomyces. . cerevisiae. ).. yeast is a living organism that requires a warm, moist environment and a food source to grow and thrive. .. unicellular, . faculative. anaerobic fungi. Homemade Yeast Bread. It has a distinctively appealing sweet smell and delicious taste.. Bread machine – measure the ingredients, and the machine does the rest. Yeast Bread Ingredients. Flour. All-purpose flour. Presented by Nathalie . Kirshman. and Xinyi Ma. Background . Protein complexes . TAP (tandem-affinity purification). Background . –. protein complexes. At the biochemical level, proteins rarely act alone; rather, they interact with others to perform cellular tasks. GLUTEN – provides dough with . elasticity. and . strength. .. YEAST – makes dough . rise. and gives it it’s porous . texture. Under developed gluten. Developed gluten. Define. HYDRATION. To Add water. “Sandwich” Bread. and…. Dinner Rolls. Cinnamon Rolls. Pizza Crust. Bagels. Croissants. Soft Pretzels. Yeast Breads vs. Quick Breads. YEAST BREADS are . NOT. quick to make . like QUICK BREADS. because they do NOT use. Joshua Egan. 10. th. . grade, Pittsburgh Central Catholic High School, 2. nd. year in PJAS . Problem. Cologne is used worldwide with unknown effects on skin microflora. Microbial Flora . Little is known about the association between humans and their flora. Raghav. . Ramachandran. Ambhi Ganesan. September 9,2008. A model for life science research. Test out various hypotheses on a smaller scale.. Saves time and money.. Practically impossible to carry out certain research directly on intended targets.. Yeast are unicellular fungi. Yeasts multiply as single cells that divide by budding (. eg.. . Saccharomyces. ) or direct division (fission, . eg.. . Schizosaccharomyces. ), or they may grow as simple irregular filaments (mycelium). . Strains: microorganisms of the same species that are descended from a single cell and possess the same genotype. What do yeast require to grow in the laboratory?. How is sterile technique used to culture microorganisms?. Beatriz Nunes Silva. , Vasco Cadavez, Pedro Ferreira-Santos, José António Teixeira, Ursula Gonzales-Barron. 1. st. International Electronic Conference on Food Science and Functional Foods. 2. Motivation.