PPT-Preparing yeast cell extracts

SDSPAGE gives a snapshot of proteins in an extract Proteins are extracted from cells There are major challenges in analyzing yeast cell proteins How can proteins be efficiently extracted without being degraded . Michelle . Attner. July 24, 2012. What is budding yeast, . S. . cerevisiae. ?. Electron micrograph. DIC (light microscopy). Advantages to budding yeast as a model organism. Simple, eukaryotic cell (~10μm diameter). Objective. Explain how dough for yeast-raised products is prepared.. Dough Preparation. Preparing dough for a yeast-raised bakery product is not as easy as preparing . doughs. and batters for quick breads. Microbiologists have developed techniques for isolating and maintaining strains in the laboratory. Strains: microorganisms of the same species that are descended from a single cell and possess the same genotype. Oxidative Stress. O. 2. is readily reduced to form one of many Reactive Oxygen Species (ROS) . ROS cause cellular damage. DNA. Protein. Lipids. http://www.vivo.colostate.edu/hbooks/pathphys/misc_topics/ros.gif. Topic 1 – Cells and DNA. Animal and plant cells. DNA, genes and chromosomes. Cell Division. DNA profiling. Topic . 2 – Microbes. !. Types of Microbes. Controlling the growth of microbes. Microbes in industry. Joshua Egan. 10. th. . grade, Pittsburgh Central Catholic High School, 2. nd. year in PJAS . Problem. Cologne is used worldwide with unknown effects on skin microflora. Microbial Flora . Little is known about the association between humans and their flora. 1-liquid . media, often . referred to . as broth. , . 2-solid-substrate fermentations.. In most industrial fermentation . processes there . are . several stages . where media are required. . 1-. . several inoculum (starter culture) . Raghav. . Ramachandran. Ambhi Ganesan. September 9,2008. A model for life science research. Test out various hypotheses on a smaller scale.. Saves time and money.. Practically impossible to carry out certain research directly on intended targets.. . Professor Alex Speers, . F.I.B.D. ., . C.Sci. .. Heriot-Watt University, Edinburgh. Oct 2015. MBAA. Conference. Jacksonville, FL. Introduction. Fermentation Overview. Flocculation. Why? ….Too much/Too little. Lythrum. . salicaria.  L.. Nikola . Sre. ćković. 1. , Jelena S. Katanić Stanković. 2. , Paola Imbimbo. 3. ,. Daria Maria Monti. 3. , Vladimir Mihailović. 1. 1 . University of Kragujevac, Faculty of Science, Department of Chemistry, Radoja Domanovića 12, 34000 Kragujevac, Serbia. Yeast are unicellular fungi. Yeasts multiply as single cells that divide by budding (. eg.. . Saccharomyces. ) or direct division (fission, . eg.. . Schizosaccharomyces. ), or they may grow as simple irregular filaments (mycelium). . Strains: microorganisms of the same species that are descended from a single cell and possess the same genotype. What do yeast require to grow in the laboratory?. How is sterile technique used to culture microorganisms?. Subject Code – . 17UMBA31. Semester – . I. V. Programme. - . B.Sc. Microbiology. . Ms.Musbira. . banu. . M. Assistant professor . Department of Microbiology. HKRH College. Mycology. (from the . Greek. . mukē. ,. meaning "fungus") is the branch of biology concerned with the study of fungi, including their genetic and . biochemical. . properties, their . taxonomy . and their use to humans as a source for .