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Tetrazolium  testing referee Tetrazolium  testing referee

Tetrazolium testing referee - PowerPoint Presentation

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Uploaded On 2024-03-13

Tetrazolium testing referee - PPT Presentation

results Southwest Region 4 2016 by Michael Aberle RST Ransom Seed Laboratory Tetrazolium testing referee results 2016 40 participants 30 laboratories 35 of the respondents were from the West coast region 4 ID: 1047978

answered viable concern viablerespondents viable answered viablerespondents concern embryo unstained kale brassica helianthus sunflower flowering scst lewisii linum flax

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1. Tetrazolium testing referee resultsSouthwest Region 42016by Michael Aberle RSTRansom Seed Laboratory

2. Tetrazolium testing referee results201640 participants 30 laboratories 35% of the respondents were from the West coast region 420 photographs8 plant families

3. 1. BLUE FLAX Linum lewisii Linaceae98% viableRespondents that answered viable:Concern that the radicle was damaged

4. 2. BLUE FLAX Linum lewisii Linaceae100% non-viable

5. 3. BLUE FLAX Linum lewisii Linaceae98% non-viableRespondents that answered viable:Concern that a membrane covered the unstained parts

6. 4. CACTUSCactaceae100% non-viable

7. 5. CACTUSCactaceae95% viableRespondents that answered non-viable:Concern that there was a crack in the embryo

8. 6. Flowering kale Brassica oleraceaBrassicaceae52% viableRespondents that answered non-viable:Concern that necrosis reached the juncture of the cotyledons and the embryo axis

9. 7. Flowering kale Brassica oleraceaBrassicaceae90% viableRespondents that answered non-viable:Concern that the radicle was brokenConcern that there was insufficient staining

10. 8. Flowering kale Brassica oleraceaBrassicaceae87% non-viableRespondents that answered viable:Concern that the necrosis is only superficial Embryo axis is not damaged Non-essential areas unstained

11. 9. Flowering kale Brassica oleraceaBrassicaceae100% non-viable

12. 10. LOVAGE Levisticum officinale Apiaceae82% non-viableRespondents that answered viable:Concern that embryo might have a light stainCould be dormant as in AOSA/SCST TZ Handbook where there is the case of an unstained embryo with stained endosperm

13. 11. LOVAGE Levisticum officinale Apiaceae100% viable

14. 12. NASTURTIUM Tropaeolum majus Tropaeolaceae95% non-viableRespondents that answered viable:Concern that the embryo stained lightly

15. 13. NASTURTIUM Tropaeolum majus Tropaeolaceae65% non-viableRespondents that answered viable:Could not relate the image to the example given in the AOSA/SCST TZ HandbookUnstained areas are minor

16. 14. OREGANO Origanum vulgareLamiaceae98% non-viableRespondents that answered viable:Saw uniform stain

17. 15. OREGANO Origanum vulgareLamiaceae100% viable

18. 16. Phormium sp.Xanthorrhoeaceae[Agavaceae]100% viable

19. 17. Phormium sp.Xanthorrhoeaceae[Agavaceae]98% non-viableRespondents that answered viable:Saw more than half the embryo stained

20. 18. Phormium sp.Xanthorrhoeaceae[Agavaceae]95% viableRespondents that answered non-viable:Concerned that the endosperm was unstained (light reflecting off the seed made it look unstained)

21. 19. SUNFLOWER Helianthus annuusAsteraceae90% non-viableRespondents that answered viable:Concern that the damage at the root tip was minimalMore than half the embryo stainedIn the AOSA/SCST TZ Handbook slight damage to the root tip is acceptable

22. 20. SUNFLOWER Helianthus annuusAsteraceae90% viableRespondents that answered non-viable:Concern over the dark spots on the radicleUnstained cotyledon

23. 21. SUNFLOWER Helianthus annuusAsteraceae100% non-viable

24. 22. SUNFLOWER Helianthus annuusAsteraceae100% non-viable