wwwehswashingtonedu April 21 2021 Page 1 of 1 BIOSAFETY LEVELS FOR VIRAL VECTORS The following table lists the biosafety levels BSLs for viral vector systems with and without oncogen ID: 938436
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Biosafety Levels for Viral Vectors │ www.ehs.washington.edu │ April 21, 2021 Page 1 of 1 BIOSAFETY LEVELS FOR VIRAL VECTORS The following table lists the biosafety levels (BSLs) for viral vector systems with and without oncogenic inserts. EH&S and the IBC will perform a risk assessment for each vector system and gene insert combination prior to approval. For RCV testing information, refer to specific information for each vector. Vector s ystem Biosaf ety l evel Biosafety level with oncogenic insert RCV testing available? Ad eno - associated viral (AAV) vector without helper virus BSL - 1/ABSL - 1 BSL - 2/ABSL - 2 No Adeno - associated viral (AAV) vector with helper virus BSL - 2/ABSL - 2 BSL - 2/ABSL - 2 Can test for presence of helper virus Adenoviral vector BSL - 2/ABSL - 2 BSL - 2 / ABSL - 2 Yes Foamyviral vector , non - integrating BSL - 1/ABSL - 1 BSL - 2 / ABSL - 2 Yes Gammaretroviral vector, amphotropic pseudotype BSL - 2/ABSL - 2 BSL - 2 with BSL - 3 practices/ ABSL - 2 with ABSL - 3 practices Yes Gammaretroviral vector, ecotropic pseudotype BSL - 1/ABSL - 1 BSL - 2/ABSL - 2 Yes Herpesvirus vector BSL - 2/ABSL - 2 BSL - 2/ABSL - 2 No Lentiviral vector, 1 st /2 nd /or unknown generation BSL - 2/ABSL - 2 BSL - 2 with BSL - 3 practices/ ABSL - 2 with ABSL - 3 practices Yes Lentiviral vector, 3 rd generation BSL - 1/ABSL - 1 BSL - 2/ABSL - 2 No Sendai viral vector BSL - 2/ABSL - 2 BSL - 2/ABSL - 2 No Sindbis viral vector BSL - 2/ABSL - 2 BSL - 2/ABSL - 2 Yes For questions, c ontact EH&S Biosafety at ehsbio@uw.edu or 206 - 221 - 7770.