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chamber allows the chamber allows the

chamber allows the - PDF document

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chamber allows the - PPT Presentation

Ra p ichi p The PCR Chip f or Ult r a F a s t r eactions Upg r ade t o a n e w f orm a t the M ic r ofluid i c Chip A Ne w PCR c o n c e p t was b o r n w h e n p r e c i s i on m o l d i ID: 819472

pcr chip rapi 00200 chip pcr 00200 rapi ker pack api channe accuthermal large heat 0mm 768 tran 384

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Rapi:chip™ The PCR Chip for
Rapi:chip™ The PCR Chip for Ultra-Fast reactions Upgrade to a new format, the Microfluidic Chip! A New PCR concept was born when precision molding technology met film bonding know how. Rapi:chip™ was developed from the understanding of the challenges in routine PCR. We found the protocols duration could be dramatically reduced when efficient heat transfer is made during the thermal cycling process. By designing a unique 3-dimensional polymer chip where the bottom surface is made of a thin film and precisely bonded with a polymer body, ultra-fast thermal cycling became possible. Options are available for both thermal cycling and real-time PCR analysis. Rapi:chip™ is supplied in two formats. The QPCR version of the chip has 10 wells and the top of each well is open for real-time fluorescence measurement. Rapi:chip/16™ is a version t which can accommodate 16 samples and is ideal for amplification of standard PCR. It does not have the capability for analysis of a fluorescence signal.Design is optimized for reducing the time for gene amplification The Flat wide chamber allows the most efficient trans- fer of heat to the sample A 10μl well volume assists in the fast heating and cooling The bottom surface of the chip is made from a thin film for efficient heat transfer Contamination and transformation free 1 & 5. Wings - These parts are used for easy handling with fingers or

tweezers. 2. Printed W
tweezers. 2. Printed Well Numbers -The Well numbers are printed on the upper surface of the chip for easy identification. 3 & 4. Apertures -Pipette tips fit in these apertures so that sample and reagent can be loaded or collected easily. How to load samples Step 1: Prepare sample according to suggested protocol. Volume of sample mixture should be 10µ Step 2: Using micropipette, aspirate prepared sample mixture (10µ) Step 3: Place Pipette tip into the inlet hole of Rapi:chip™ and dispense the entire volume of the aspirated sample Step 4: Seal all holes using the enclosed sealing tape, rub the surface of the sealed area to secure the sealing Cat. No. Description 002001 Rapi:chipTM PCR Chip for GENECHECKER® – Small Pack Specifications Rapi:chipTM and Rapi:chip/16TM PCR Chip for GENECHECKER® Dimension Weight Number of Wells Volume of Each Well Aperture Diameter Channel Height Channel Length Channel Width Material Transparency 38mm (w) x 25mm (d) x 6 mm (h) 3.56 g 10 Wells (Rapi:chipTM), 16 Wells (Rapi:chip/16TM) 10 µL 0.6mm 0.5mm 8.0mm 2.0mm Transparent Polymer More than 95% Ordering Information Pack 48/PK 002002 Rapi:chipTM PCR Chip for GENECHECKER® – Medium Pack 384/PK 002003 Rapi:chipTM PCR Chip for GENECHECKER® – Large Pack 768/PK 002004 Rapi:chip/16TM PCR Chip for GENECHECKER® – Small Pack 48/PK 002005 Rapi:chip/16TM PCR Chip for GENECHECKER® – Medium Pack 384/PK 002006 Rapi:chip/16TM PCR Chip for GENECHECKER® – Large Pack 768/PK 003001 Car Connection Power Kit 1 SET Accurate Thermal Systems 4104 Sylon Blvd, Hainesport, NJ 08036 Phone: 609-326-3190 email: sales@accuthermal.com web: accuthermal.com