PDF-JOURNALOFBACTERIOLOGY,June1980,p.962-9720021-9193/80/06-0962/11$02.00/

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pol4lacZGENEFUSIONS963resisin1agarosegels47DNAfragmentswerejoinedbyusingT4polynucleotideligaseandrecombinantmoleculesrecoveredbytransfectionofstrainAA125madecompetentintheuptakeofDNAbyaslightmod

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JOURNALOFBACTERIOLOGY,June1980,p.962-9720021-9193/80/06-0962/11$02.00/: Transcript

pol4lacZGENEFUSIONS963resisin1agarosegels47DNAfragmentswerejoinedbyusingT4polynucleotideligaseandrecombinantmoleculesrecoveredbytransfectionofstrainAA125madecompetentintheuptakeofDNAbyaslightmod. AversionofthispaperwaspresentedattheBabson/KauffmanEntrepreneurshipResearchConference,Wellesley,MA,June2000.*Correspondingauthor.Tel.:+1-651-962-4407;fax:+1-651-962-4410.E-mailaddresses:wgartner@marsh 904NOTES0041.00.80.610.40.2246369TIME,miin.[INHIBlTOR,jiMIFIG.1.(A)Octopine(LI)andnopaline(A)transportbyA.tumefaciensA136.Theinitialconcentrationofopineswas1,uMinbothcases.(B)Plotsofrelativerateofupta 123 ISSN 0962-7286 FEATURES/BENEFITS Remote lockout input combine an AquaMatic stager with a 962 series electronic control, mounted and prewired in a NEMA-rated enclosure Can be used simultaneously with time clock, 4064NOTES*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~.............l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~...l............FIGElctomirogapoP.puidPR20.Bar,1..view.Cellswithapathlengthoflessthan30p.mwerenotanalyzed.Pathsofce &. General Fund Budget. Proposed 2017 Tax Levy. Increase of $63,349 from 2016 tax levy. (+5.7%). All non-enterprise fund debt service is covered.. Tax rate would reduce for third straight year (-1.433%).. IMMUNOGENICRIBOSOMALANDRNAPREPARATIONSMATERIALSANDMETHODSMycobacterialstrains.TheattenuatedH37RastrainofMycobacteriumtuberculosiswasusedasthesourceofallmycobacterialribosomalandRNAfrac-tions.Thisstrai 3464GONCHAROFFETAL.korAkorAkorBkorAkorAkorAkorBkorAkorAkorBkorEkorCkokorCkorEkorBkorFkilBtrifAoriVkiCkorCkilEkIdAkorAkorBkorFkIrA............TcrkorE#Ter/fiwBI.kilApkilAHi2.4'incCkp.,,XEHi056.4'55.7-FI GENETICCONTROLOFPERSISTENCE769ofpersistence(Hipmutants),aconvenientmethodforscoringtheHipphenotype,themap-pingofhipAbyconjugationandtransduction,andtheinitialcharacterizationofisogenichipAandhipA+stra E.COLIp)orU7t)67TABLE1.Bacteria,bacteriophages,andplasmidsusedinthisworkStrain,phage,orplasmidGenotypeordescriptionSourceorretereinceE.coliMC4I)(F-araDl39A(argF-/ac)U169rpsL150thiAIre/AIdeoCiptsF25flb SUPPRESSIONOFColElHIGH-COPY-NUMBERMUTANTS429gyrA96relAl]wasusedastheplasmidhostintheseexper-iments(8).StrainsusedforHfrmappinganddonorstrainsforPlvirtransductionweregiftsfromC.A.Gross,Univer-sityofWis BINDINGTOCOLLAGENBYYERSINIASPP.6675TABLE1.Bindingof'25I-labeledcollagenbyYersiniaspp.andE.colistrainsPresenceofPresenceof%bindingofcollagenSourceorOrganismSerogroupvirulenceYOP1SplasmidexpressionType1 TABLE1.Responsesofvolunteerstoinoculationswithwild-typeHINIinfluenzaAviruses(serotypeAlUSSR/90/77)"VirusNo.ofNo.withthefollowingclinicalAvgNo.ofNo.ofAvgdosevirusreactions:'scoreofNo.ofViruseggpas-volu 192KOPLINETAL.TABLE1.BacterialstrainsandplasmidsStrainorplasmidRelevantcharacteristicsReferenceStrainsE.coliS17.1E.coli294,RP4-2-Tc::Mu-Km::Tn752X.campestris27B100DSM1526,Smr27B100-152SpontaneousEPS-m

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