PDF-JOURNALOFBACTERIOLOGY,July1969,p.42-50Vol.99,No.tCopyright@1969America

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IMMUNOGENICRIBOSOMALANDRNAPREPARATIONSMATERIALSANDMETHODSMycobacterialstrainsTheattenuatedH37RastrainofMycobacteriumtuberculosiswasusedasthesourceofallmycobacterialribosomalandRNAfractionsThisstrai

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JOURNALOFBACTERIOLOGY,July1969,p.42-50Vol.99,No.tCopyright@1969America: Transcript

IMMUNOGENICRIBOSOMALANDRNAPREPARATIONSMATERIALSANDMETHODSMycobacterialstrainsTheattenuatedH37RastrainofMycobacteriumtuberculosiswasusedasthesourceofallmycobacterialribosomalandRNAfractionsThisstrai. 842ZANKERETAL.A.Qregion3540(kbp)IIaAeI1m---------------------------_,E211ENENiIiocciB.nocregion510------leftpart---------HNEKEElgEd9am~u&am heap cheep ch high chai ch hook chook The aspiration trick for /tCopyright 4064NOTES*~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~.............l~~~~~~~~~~~~~~~~~~~~~~~~~~~~~...l............FIGElctomirogapoP.puidPR20.Bar,1..view.Cellswithapathlengthoflessthan30p.mwerenotanalyzed.Pathsofce 974ORREGO,ARNAUD,ANDHALVORSONTABLE1.StrainsusedB.subtilisGenotypeSourceandcomments168trpC2OurstockANS-1Spontaneousrevert-Ourstockantof168BC109trpC2thyAthyBJ.Copeland(F.Roth-man,initialsource)BC50purA1 3464GONCHAROFFETAL.korAkorAkorBkorAkorAkorAkorBkorAkorAkorBkorEkorCkokorCkorEkorBkorFkilBtrifAoriVkiCkorCkilEkIdAkorAkorBkorFkIrA............TcrkorE#Ter/fiwBI.kilApkilAHi2.4'incCkp.,,XEHi056.4'55.7-FI 1AAGCTTATTATGCTTCCATCGGAAACAATGATCTGGCAGCCTGAGTTCACAGATAAAATATCTCTCCAGGAAACCCGGGGCTGTTCATCATGCAAGTCTG*(-35).(-10)(SD)3rafERK101TCGATTACTGGCTGGTGACGGAATTTTCTGGATTTCCGGCTTAGAACCACAGCAGGAGATAATATGTCACTTA pol4-lacZGENEFUSIONS963resisin1%agarosegels(47).DNAfragmentswerejoinedbyusingT4polynucleotideligaseandrecom-binantmoleculesrecoveredbytransfectionofstrainAA125madecompetentintheuptakeofDNAbyaslightmod BINDINGTOCOLLAGENBYYERSINIASPP.6675TABLE1.Bindingof'25I-labeledcollagenbyYersiniaspp.andE.colistrainsPresenceofPresenceof%bindingofcollagenSourceorOrganismSerogroupvirulenceYOP1SplasmidexpressionType1 852NOTESTABLE1.IsolationofyeastmutantsunabletoutilizearginineasthesolenitrogensourceDeterminationNo.Colonieson100phloxineBplates'........27,000Smallredcoloniestested.................743Respiratory-def

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