PDF-Proc.Nati.Acad.Sci.USAVol.91,pp.7159-7163,July1994DevelopmentalBiology

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7160DevelopmentalBiologyPavanandTilghmanoverthehindlimbsAtleasttwoembryosofeachgenotypewereexaminedforeachtimepointEmbryosweresectionedat10umandtransferredtoSuperFrostPlusmicroscopeslidesFisherSc

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Proc.Nati.Acad.Sci.USAVol.91,pp.7159-7163,July1994DevelopmentalBiology: Transcript

7160DevelopmentalBiologyPavanandTilghmanoverthehindlimbsAtleasttwoembryosofeachgenotypewereexaminedforeachtimepointEmbryosweresectionedat10umandtransferredtoSuperFrostPlusmicroscopeslidesFisherSc. Proc.Natl.Acad.Sci.USA87(1990)6389Corey-Bransona-helixcannotberepresentedwithperfectaccuracyonanylatticeoflowcoordinationnumber,butthehelicesobservedincrystallographydatabasesofrealpro-teinsarelikewis Proc.Natl.Acad.Sci.USA87(1990)wereprepared(25),andtheDNAinsertwasexcisedwithEcoRI,isolatedbylowmeltagarosegelelectrophoresis,andrandom-primedforexpressionanalysis.Theexpressionlev-elsandsizesofthemRNA Proc.Nat.Acad.Sci.USA72(1975)hasalowerboundPI(tY1t+h)n(Y-&#x Tj ;&#x/F1 ;.9;� Tf;&#x 33.; T;&#xz -0;&#x.24 ;&#xTs 1;.10;&#x 0 T; 00;t+h*)1&#x Tj ;&#x/F1 ;.9;� Tf;&#x 33.;& MedicalSciences:Cherif-Zaharetal.Proc.Natl.Acad.Sci.USA87(1990)6245aatcccggcctgcacagagacggacacaggATGAGCTCTAAGTACCCGCGGTCTGTCCGG60MetSerSerLysTyrProArgSerValArg9CGCTGCCTGCCCCTCTGGGCCCTAACACTGGAAGCAGCTC Proc.Natl.Acad.Sci.USA93(1996)Table1.PNDsdefinedbyautoimmuneantibodiesNeurologicsyndromeParaneoplasticnamePhenotypeTumorAntibodyCerebellardegenerationPan-cerebellardysfunction(Purkinjecell)TypicalBrea 868CellBiology:OsbornandWeberFIGS.1-7.(Legendappearsatbottomofthefollowingpage.)Proc.Nat.Acad.Sci.USA73(1976) Proc.Nat.Acad.Sci.USA73(1976)869FIG.8.Recoveryofcytoplasmicmicrotubulesin3T3cellsafterColc Proc.Natl.Acad.Sci.USA93(1996)11529BothLandL*canbeprotonatedonLys-156,butthepKaforprotonationisconsiderablylowerforL*thanforL;pKL*iswhilepKLis10.OneimportanttenetofthismodelisthattheIndsmutationsincre I,fromFlavobacteriumokeanokoites,isamemberoftheunusual,typeIIsclassofrestrictionendonucleasesthatrec-ognizeaspecificDNAsequenceandcleavenonspecificallyashortdistanceawayfromthatsequence(1).Ibindstheco TheNunproteinofbacteriophageHK022isamemberofthearginine-richmotiffamilyofRNAbindingproteins,whichincludesthephageNtranscriptionantiterminationproteinandtheHIVTatandRevproteins(Fig.1;refs.1±3).Nunbind Proc.Natl.Acad.Sci.USA88(1991)5593Table1.YeaststrainsNuclearMitochondrialStraingenotypegenotypeRef.947aade2ura3-52p09ID41-6aadellysi15161p+15C1036mit-;COXJintron116splicing-defectivepointmutant(trans- Cellsrespondtodevelopmentalcuesandenvironmentalstimulibyalteringgeneexpression.Often,transmissionofdifferentupstreamsignalsinvolvescommoncomponentsyetelicitsdistinct(butappropriate)outcomes.Numerousag Proc.Natl.Acad.Sci.USA93(1996)2583multiplexamplificationasdescribedabove.Theonlydiffer-encebetweenthesetwoproceduresisthatsomePprimersusedinMGSmaycontainsingle-basemismatchestothetemplatestoconvertthe Proc.Natl.Acad.Sci.USA88(1991)downstreamofthelacpromoterasfollows.PlasmidpHS202(5),whichcontainsnucleotides1-1010(thePvuII-KpnIchromosomefragmentthatencompassesruvA)intheSmaI-KpnIsiteofpUC19,wasdigest 2612Biochemistry:Sancaretal.PstIHaelilt*.JHinfl-TaqIadzJHpa11ti.r4HhaI'recAmRNA-recAprotein-Proc.Nati.Acad.Sci.USA77(1980)EcoRI-9A.-ii4------200020040060080010001200BasepairsFIG.1.Restrictionmapandseq

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