PPT-Assessing the quality and yield of nucleic acids PART 2: gel electrophoresis

1 The spectrophotometer cannot tell you if your DNA or RNA are intact undamaged Vigorous shaking pipetting up and down and vortexing can damage DNA breaking it into smaller pieces To be sure it is not damaged you can run a small amount 100200 ng on a gel . Electrophoresis. • . Separation technique based on the movement of . analyte. through a conductive medium in response to an applied electrical field.. • . The medium is usually a buffered aqueous. THE STUFF OF LIFE. Biomolecules. Organic molecules made up of:. Carbon. Hydrogen. Nitrogen. Oxygen. a. nd sometimes Phosphorus. LATIN 101. Poly = . Many. Mono = . O. ne. Hydro = . Water. . (actually Greek). 36. Which . of the following statements best describes the structure of DNA. ?. A. . Two strands of proteins are held together by sugar . molecules. , . nitrogen bases, and . phosphate groups.. B. . 1-deoxyribonucleic acid (DNA). 2-Ribonucleic acid (RNA. Nucleic. Acids. Structure: Nucleotide. Monomer : . Nucleotide. A pentose sugar molecule .. 2.A phosphate group .. 3.A nitrogenous base .. Monomer : . Biomolecules. Organic molecules made up of:. Carbon. Hydrogen. Nitrogen. Oxygen. a. nd sometimes Phosphorus. LATIN 101. Poly = . Many. Mono = . O. ne. Hydro = . Water. . (actually Greek). Synthesis = . Biochemistry-Macromolecules 4 types Carbohydrates Lipids Proteins Nucleic Acids Carbohydrates Function of Carbohydrates Energy source!!! Energy is stored in the C-H bonds Plant Structure Cellulose is a carbohydrate found in the cell wall of plants By:. . Preeti. . S. . Salve. KLE . College of Pharmacy, . Belagavi. A constituent Unit of KLE Academy of Higher Education and Research. Nehru Nagar, . Belagavi. . –. 590 010, Karnataka, India. Phone:. Pgs.:. 9-10. Heading: . Nucleic Acids. Homework:. . SILENTLY complete the Do First and glue on page 9. SILENTLY update notebook. Do First. DO FIRST (PG 9). Which biomolecule…. Makes up hair, nails, and feathers? c. Provides cells with cushioning. , Function & Repair . Lecturer 7 . Dr. . . Shaimaa. . Munther. . Organelles of the cell. . . Living organisms are made up of cells Each of these cells have a viscous substance called . cytoplasm. Spectrophotometry. 1. Assessing the Quality and Yield of Nucleic Acids:. Ultraviolet (UV) Spectrophotometry. By measuring the amount of light absorbed by your sample at specific wavelengths, it is possible to estimate the concentration of DNA and RNA. Nucleic acids have an absorption peak at ~260nm.. BSc 3. rd. year . AMINO ACIDS. C. lassification of amino acids . Classification according to nature of side chain. PREPARATION OF AMINO ACIDS. 1. Gabriel Phthalimide synthesis. 2. Strecker’s synthesis. B2 - Compare the . structures. & . functions. of carbohydrates,. . lipids, proteins, & . nucleic acids . & . their organization into long complex molecules . Consist of:. . Carbon. , hydrogen, oxygen, . Lecturer 7 . Dr. . Shaimaa. . Munther. . General. . Introduction. . Nucleic acids are required for the storage and expression of genetic information. . There are two chemically distinct types of nucleic acids: (. - DNA & RNA. Types of Nucleic Acids. –. Virus with . ssDNA. Virus with . dsDNA. Virus with . ssRNA. Virus with . dsRNA. Virus with single stranded cyclic DNA. Virus with double stranded cyclic DNA.