PPT-Extraction of DNA, RNA and Protein from FFPE samples using AFA Technology

Martina Werner June 2019 Introduction Formalin Fixed Paraffin Embedded FFPE tissue samples are widely used in clinical and research settings The sample preservation enables histological analysis Clinical labs process large numbers of cancer samples. Chapter 13 . (Pgs 360-389 Miller and Levine Biology text. ). http://www.youtube.com/watch?v=suN-sV0cT6c. . Where have we seen DNA being replicated?. MITOSIS AND . MEIOSIS. Proteins are molecules that are necessary for virtually every activity in your body. Some of the Biological Functions of Proteins include:.  RNA and Protein Synthesis. Genes. are coded . DNA. instructions that control the production of . proteins. . DNA is like the master plan.. RNA. contains coded information for making proteins.. RNA . CHM . 341. Suroviec. . Fall . 2016. I. . Nucleotides, Nucleic Acids and Bases. Bases. Planar, aromatic, heterocyclic. Purine (2 rings). Pyrimidine (1 ring) . Adenine (A). Guanine (G). Thyamine (T). From DNA to Proteins. 2 Types of nucleic acid. DNA- Life’s Code. DNA. -> . RNA . -> . Protein. Influential Scientists. Frederick. Griffith. – Experimented with two forms of . bacteria. From DNA to Proteins. 2 Types of nucleic acid. DNA- Life’s Code. DNA. -> . RNA . -> . Protein. Influential Scientists. Frederick. Griffith. – Experimented with two forms of . bacteria. Chapter 12 DNA & RNA I.DNA A. Griffith & Transformation Frederick Griffith was trying to figure out how bacteria made people sick-how they cause a certain type of pneumonia. He isolated 2 strains(types) from mice-both cultured well,but only one caused pneumonia.The culture of the disease causing bacteria were __________________colonies while the other was rough Transformation. 1928- . Frederick Griffith . was trying to figure out how bacteria made people sick. Pneumonia. Cause of pneumonia was not a chemical poison released by disease causing bacteria. The heat killed bacteria had passed their disease-causing ability to the harmless strain. Chapter . 17.1-17.3. Important Terms to Know…. 25% of students confuse these terms in . FRQs!!. Replication. Copying of DNA . prior to cell division. (DNA .  DNA). Not prior to transcription. !. Intro. The genes in DNA code for instructions that control the production of proteins within the cells. The first step in decoding these genetic messages is to copy DNA into . RNA (transcription). These RNA molecules contain coded information for making proteins. . From Specimen Lysis to NGS and LCMS Analysis . APAC 2019. www.covaris.com. Sample Prep. The current bottleneck in High Throughput Genome Analysis. NGS Workflow with current non-multiplexed Library Prep. What organelle makes proteins?. Where are these organelles found?. How does the information from DNA get to those organelles?. Protein Synthesis Overview. The information in . DNA . is used to make . Review…. DNA is responsible for controlling the production of proteins in the cell, which is essential to life. DNA. RNAProteins. Chromosomes . contain several thousand . genes. , each with the directions to make . TRIzol. purification: precipitate out everything BUT the RNA. Two common methods for separation of RNA from DNA. Selective precipitation of RNA using Lithium Chloride (LiCl). Extraction with phenol buffered at an . cells. ?. It . is not possible for protein to be made from DNA directly because DNA cannot leave the . nucleus.. It . allows for more levels of control for protein . synthesis.. mRNA . is more flexible and single stranded while DNA is double-stranded and helical, and therefore its strands cannot easily be .