Radio Frequency Cancer - PowerPoint Presentation

Slide1

Radio

Frequency

Cancer

Treatment

Muhammad Bhatti,

Juan

Lopez,

Megan

Keniry

University

of

Texas

Rio Grande

Valley

Edinburg,

Texas

,

USA

Slide2

Abstract

The

primary objective

of this research

endeavor is to study and

to understand the natural physics phenomenon of electromagnetic resonance

in

one

end

closed

cavity

for the

eventual

purpose of

cancer

treatment.

Radio

Frequency

waves

are discharged into

a

coaxial

cavity

filled with

a

small amount (1.6

mL)

of breast cancer

cells

(BT549) and

the reflection

as

well

as

the

power

input

is measured to

determine

the absorption

power

into

the

vitro

cancer

cell

experiment.

Slide3

Conceptual

outlook

All matter contains natural

frequency of

oscillation

Such as a goblet resonating

at

natural

frequency

Once

goblet

reaches

natural

frequency,

goblet

self

destruct’s

Cell

can

be

oscillated

at

its

natural

frequency

Cell

can

be

destroy

by

reaching

resonance

frequency

Destruction

of cells

can

be

selective

Slide4

Introduction

We

are interested in experimenting with

electromagnetic radiation in the

radio wavelength and

cancer cells. We are looking for

ways

to kill cancer

cells using

RF

while keeping

the

maximum

possible

number

of

healthy

cells

alive.

Slide5

Method

ology

Coaxial cavity

with one end

closed

An PASCO Capstone Interface is then

used

with

software

A frequency

signal

Generator is

then operated

to

generate

833

MHz

Add 1.6

mL

sample

that

is being loaded into

the

waveguide

Input

wave

and

reflected

wave

powers

are recorded with

power

sensors

Slide6

Equipment

Setup

Slide7

Temperature

Gradient

Slide8

T

heory

Free vibrations of an elastic body are called

natural

vibrations

occur at a frequency called the natural

frequency.

If

forced frequency

is

equal to the

natural

frequency,

the amplitude

of

vibration

increases many

times,

this is

known

as resonance

phenomenon.

Determine

the

reflected

wave

is

at

its

minimum

which

is

a

condition

for

the

cavity

resonance.

Slide9

T

heory

Cell are

made

of moving atoms,

the vibrations inside cells travel as

waves,

at an

approximately

constant

velocity,

bouncing

back

and

forth

between

the sides

of

the

cell

membrane.If the length of the wave guide is L. To cause resonance, the phase ofa sinusoidal wave after a roundtrip must be equal to the initial phase, so the waves reinforce the oscillation or when absorption reaches maximum.

• 𝑓 = 𝑁𝑣

4 𝐿

𝑁𝜖

1

,

3

,

5

…

Slide10

Experimental

Purpose

To determine

the effect of radio frequency

electromagnetic waves on human cell

cultures

in

vitro

cytotoxicity

testing

was

carried

out using human breast cancer cell

line

BT549 and human embryonic

healthy

kidney 293 cells

(HEK-293)

Slide11

Cell

Preparation

The Trevigen

TACS MTT

Cell Proliferation Assay Kit

(Gaithersburg, MD) was utilized to assess

impacts on human cell proliferation and/or

viability

Cells were

grown under

standard tissue culture conditions

(5%

CO

2

,

37°C,

with 10%

FBS

and

pen/strep)

Cells were removed from plates with, centrifuged at 25°C (100 x g for 10 minutes) and re-suspended in fresh media at a density of 250,000 cells per mL prior to treatment

Slide12

Cell

Treatment

Cells were treated at

833 MHz frequency

Cells

were in a control

environment

Total

time of exposure

was

20

minutes

Voltage

was

at

11

volts

for the

amplifier

input

Amperage of 0.62 amps being used by the amplifierControl mass of about 1.5 g

Slide13

Cell

Analyzes

After treatment,

100 mL from cell suspensions

were plated into wells

of 96 well plates (each sample

was

assessed

in

quadruplicate)

After an

18

hour

recovery

(5% CO

2

,

37°C),

10 mL

of MTT

reagent

was added to each well and incubated (5% CO2, 37°C) for four hours.100 mL of detergent reagent was added to each well and samples were incubated (5% CO2, 37°C) for four additional hoursOD 590

was measured to detect MTT reduction to formazan dye (by mitochondrial enzymes) in order to assess

cytotoxicity

Slide14

R

esults

Slide15

O

D

59

5

n

m

1.2

1

0.8

0.6

0.4

0.2

0

Time=

0

Time=

1

Time=

2

Tim

T

e

im

=

e

3

in

T

h

i

o

m

u

e

rs

=

4 Time = 6

Time=

7

Time=

24

Control

BT549 experiment

with

cell left out of incubator

4.8.16

Slide16

11.24.16

experiment

BT549

Basal

breast cancer

cells

Slide17

Conclusive

results

0

.7

0

.6

0

.5

0

.4

0

.3

0

.2

0

.1

0

C

o

n

t

ro

l

833,

v=11

883,

v=10.5

833,

v=9

O

D

59

5

n

m

Slide18

0

0

.1

0

.2

0

.3

0

.4

0

.5

0

.6

0

.7

0

.8

293

control

293

833

293

833

O

D

59

5

n

m

BT549

and

HEK

203 control

cells

6.20.16,

v=12.5

Slide19

0

0

.2

0

.4

0

.6

0

.8

1

1

.2

1

.4

BT549

control

BT549

833

BT549

833

O

D

59

5

n

m

BT549

and

HEK

203 control

cells

6.20.16,

v=12.5

Slide20

11.24.16

experimentBT549 Basal

breast cancer cells

Slide21

Discussion

These

results help us

understand that if the intensity of the frequency

is lowered, more cells might die. The test conducted

at11.0 Volts

to

amplifier resulted

in killing

of

the

BT549

breast

cancer

cells

(approx.

51%)

in the waveguide.

Additionally,

the

test

conducted at

11.0V resulted on 30% of the Kidney Cells 293. This suggests that wave amplitudes must be selected carefully for tests, and that the healty kidney cells were much more resistant to the experiments. More tests of cytotoxicity/viability are underway.

Slide22

Future

Research

Our future

goals

will

include alternating frequencies, voltage, and treatment duration

to

eradicate as

many

cells as possible.

We

will

also

investigate

how

to

specifically target the

cancer

cells

only

without

damaging the healthy cells. Additional cancer cell lines

will be investigated as well as more carefully designed experiments will be conducted. Possibility of caner cell death from thermos will be reduced.

Slide23

R

eferences

1.

K

enir

y

,

M

.

an

d

R

.

P

arsons,

T

he

r

o

l

e of PTEN signalingperturbations incancer and in targeted therapy. Oncogene, 2008. 27(41): p:5477-852. Markov,

M.S., Expanding use of pulsed electromagnetic field therapies.

Electromagn. Biol. Med, 2007. 26(3): p-257-74

3. Lsciacero, P., et al., The role of radiation therapy in vulvar cancer. Review ofthe current literature. Tumori. 2016: p..4. Mu,Y., et

a

l

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, E

f

f

ec

t

i

veness

an

d

safe

t

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o

f

chemothera

p

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t

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cytokine-induced killer

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killer

cell

therapy

f

or

t

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m

ent

o

f gast

r

i

c

canc

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r

i

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C

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