PPT-GENE CLONING TOOLS

Gene Cloning allows the separation and identification of a specific section of genetic material DNA or RNA from other sequences It then allows the isolation of large numbers of copies of . (Part 1). Created by: Haley . Vrazel. Objectives. Define cloning.. Identify the history of cloning. . Analyze the different types of cloning.. What is Cloning?. Animal cloning is an assisted reproductive . (Part 1). Created by: Haley . Vrazel. Objectives. Define cloning.. Identify the history of cloning. . Analyze the different types of cloning.. What is Cloning?. Animal cloning is an assisted reproductive . Son. Genetic Engineering. It is the direct manipulation of an organism’s genome using modern DNA technology … also called genetic modification. A genome is the entirety of an organism’s hereditary information. Gene Cloning . allows the separation and identification of a specific section of genetic . material . (DNA or RNA) from other sequences. . It then . allows the isolation of large numbers of copies . of . A number of different processes used to produce genetically identical copies of biological material. Researchers have cloned genes, cells, . tissues, and entire organisms. Do clones ever occur naturally?. 1. Recombinant DNA . molecules are formed when segments of DNA from two different sources—often different species—are combined . in vitro. . . DNA . Technology . is the term. . for analyzing genes and gene expression. . Gene cloning and other techniques, collectively termed DNA technology, can be used to manipulate and analyze DNA and to produce useful new products and organisms.. In genetic engineering, bacterial restriction enzymes are used to cut DNA molecules within short, specific nucleotide sequences (restriction sites), yielding a set of double-stranded DNA fragments with single stranded sticky ends.. Restriction Enzymes. Gel Electrophoresis. PCR. Vectors/plasmids. Gene Library/Microarray. Stem Cells. DNA sequencing . DNA Cloning. What is it?. What is it used for?. How does it work/what does it do?. Principle, Requirements, steps and applications Dr. Sudha Kumari Department of Veterinary Microbiology Bihar Veterinary College, Patna Bihar Animal Sciences Un DNA Cloning . DNA cloning. . is process of making several identical copy of a gene or gene fragment. . Cloning. is a . natural process. . in biology where genetically identical individuals are produced by asexually reproducing organisms such as bacteria, insects or plants. In . Rubanraj. . Dept of Mathematics. St. Joseph’s College. Trichy. Vectors . Vector is an agent that can carry a DNA fragment into a host cell in which it is capable of replication. If it is used only for reproducing the DNA fragment, it is called a . HOD Botany. MD College. Parel. ,. Mumbai-12 . Gene Cloning. Gene cloning.  is the process in which a . gene.  of interest is located and copied (. cloned. ) out of DNA extracted from an organism. . is the production of identical copies of organisms, cells or DNA. . A . clone. is a genetically identical organism or a group of genetically identical cells derived from a single parent.. Cloning occurs in nature as part of growth and reproduction. This natural process can be manipulated to produce clones of organisms artificially.. Biotechnology: Recombinant DNA and Cloning. Module 1 presentation B. Biotechnology: . Recombinant. DNA and Cloning. Learning intention. Describe the process of making recombinant DNA. Success criteria .