PPT-Adsorption, ion exchange, and chromatography

1 Introduction Sorption operations certain components of a fluid phase called solutes are selectively transferred to insoluble rigid particles suspended in a vessel or packed in a column. PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . Chromatography. Chromatography is a technique of separation of different dissolved constituents of a mixture by absorbing them over an appropriate adsorbent material .It is a versatile method of separating different kinds of chemical mixtures .. Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . Adam . Hasham. , Sam . Heavenrich. , . Jayanth. . Prakhya. , Enoch . Tieu. Chromatography. Separates mixtures (physical method). Involves:. Stationary Phase; Surface. Mobile Phase; Solvent . Quantifiable:. Basics of adsorptive therapy . “Yesterday, I couldn’t define colloid chemistry; today, I’m doing it. .”. From Abbott’s Flatland. Colloidal Chemistry. A colloid is a substance microscopically dispersed evenly throughout another . Dr. Kevin Ahern. Protein Purification. Applications of Biochemistry Knowledge. Opening Cells. Centrifugation. Fractionation. Dialysis. Applications of Biochemistry Knowledge. Separates salts from proteins. of . Reactive . Yellow-145 Dye on Iraqi Date Palm Seeds Activated Carbons. . Falah. H. Hussein⃰, Abbas J. . Lafta. , Ahmed F. . Halbus. , . Zaineb. S. . Adsorption, ion exchange, and chromatography 1 Introduction Sorption operations: certain components of a fluid phase, called solutes , are selectively transferred to insoluble, rigid particles Dr.Methak. A . ljboori. Ion Exchange Chromatography. Ion exchange chromatography (IEX) is a technique that is commonly used in . biomolecule. purification. It involves the separation of molecules on the basis of their charge. By . Dr. . Amjad. M. T. Khan. Assistant Professor. Department of Chemistry. AMU, Aligarh. CONTENTS. Introduction to chromatography. History. Principles. Importance. Chromatographic terms. Classification of chromatography. The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Definition. Ion-exchange chromatography. (or . ion chromatography. ) is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. . 1975 by Small, Stevens and Baumann. . . CHROMATOGRAPHIC TECHNIQUES. CONTENTS. Introduction to chromatography. History. Principles. Importance. Chromatographic terms. Classification of chromatography. Adsorption chromatography. Partition chromatography.