PPT-CHROMATOGRAPHY 1 Biochemistry of Medics

The word Chromatography is derived fro two Greek words Chroma means colour and graphein to write Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. Over the past decade an ever growing arsenal of techniques has helped researchers dissect the innermost secrets of the cell and develop new ways to detect and attack disease These techniques also have been used to produce vast amounts of once rare d PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. AP Investigation 5: Photosynthesis – Part I. Chromatography. T. echnique . used to separate a mixture into its component . molecules. The . molecules migrate, or move up the paper, at different rates because of differences in solubility, molecular mass, and hydrogen bonding with the paper. Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Lecture 7 a Chromatography I Introduction Chromatography was discovered by Russian botanist Mikhail Semyonovich Tsvett , who separated plant pigments using calcium carbonate columns (1901) Martin and Synge ( GC . is currently one of the most popular methods for separating and analyzing compounds. . GC can be applied to the separation of any compound that is either naturally volatile (i.e., readily goes into the gas phase) or can be converted to a volatile derivative.. 1. Layla. is a 35 year old woman is seen for easy fatigue for many months. She is now 24 weeks pregnant with her 3rd child in 3 years. She does not see any obstetrician and does not take any vitamins. Lately, she has developed a taste for eating ice. She has no other complaint. Family and past history are negative. Physical examination is positive for pale conjunctiva, mild spooning of nails, and a II/VI systolic murmur at left lower sternal border. Her B.P is 100/60 .Stools are negative for occult blood. Labs: Complete blood count (CBC) - . 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University. INTRODUCTION: GAS CHROMATOGRAPHY . Origin of gas chromatography: 1905, W. Ramsey . Separated mixture of gases and vapors . Used solid adsorbent: activated charcoal. . A sample containing the materials to be separated is injected into the gas chromatograph. . Gas Chromatography. A mobile phase (carrier gas) moves through a column that contains a wall coated or granular solid coated stationary phase. . Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . Definition. Ion-exchange chromatography. (or . ion chromatography. ) is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. . 1975 by Small, Stevens and Baumann. MLA project B.PBE.0037 . Phil Nichols. Department of Agriculture and Food Western Australia. The project team. What we did. Developed new tools & technologies to increase rate of genetic gain in annual legume breeding.