PPT-Plant Pigment Chromatography

AP Investigation 5 Photosynthesis Part I Chromatography T echnique used to separate a mixture into its component molecules The molecules migrate or move up the paper at different rates because of differences in solubility molecular mass and hydrogen bonding with the paper. Supervisor:. Anton Zavialov. By: . Elham Barazeghi. Mehrafarin Ramezani. Affinity Chromatography:. Separation of proteins by reversible interaction of proteins and specific ligand in matrix of column. Shera Casseus. Purpose of Lab. The purpose of the Pigment/Paint lab is to create pigments for painting using transition metal compounds.. . Vocabulary . Pigments. - Used for coloring paints, ink, clothing and food. Pigments gets their color from transition metals.. Invention of Chromatography. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . BP Schools Link. Looking for Chemicals which are hidden . BP Schools Link – . Creative Chromatography. 8. In some products the chemicals we want to measure can be hidden / masked by other bigger molecules. The next two (2) experiments introduce Gas Chromatography and Simple & Fractional Distillation. This Week. Gas Chromatography – Acetates. Pavia – p. 817 - 836. Slayden – . p. 39 - 31. . Hazards and Controls. Flash Chromatography. Hazard . Control. Spilled solvent could contact skin. Solvent could penetrate through gloves and contact skin . Wear goggles, gloves (nitrile), and lab coat. Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Mikhail . Tswett. , Russian, 1872-1919 . In 1906 . Tswett. used chromatography to separate plant pigments, He called the new technique . chromatography. . because the result of the analysis was . 'written colors” . GC . is currently one of the most popular methods for separating and analyzing compounds. . GC can be applied to the separation of any compound that is either naturally volatile (i.e., readily goes into the gas phase) or can be converted to a volatile derivative.. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University. INTRODUCTION: GAS CHROMATOGRAPHY . Origin of gas chromatography: 1905, W. Ramsey . Separated mixture of gases and vapors . Used solid adsorbent: activated charcoal. . A sample containing the materials to be separated is injected into the gas chromatograph. . Gas Chromatography. A mobile phase (carrier gas) moves through a column that contains a wall coated or granular solid coated stationary phase. . Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. Definition. Ion-exchange chromatography. (or . ion chromatography. ) is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. . 1975 by Small, Stevens and Baumann.