PPT-Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography

Instrumental Analysis Instrumentation Paper Chromatography GC Gas Chromatography HPLC High Performance Liquid Chromatography IR Infrared Spectroscopy UVVis Spectroscopy MS Mass Spectrometry. Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. AP Investigation 5: Photosynthesis – Part I. Chromatography. T. echnique . used to separate a mixture into its component . molecules. The . molecules migrate, or move up the paper, at different rates because of differences in solubility, molecular mass, and hydrogen bonding with the paper. BP Schools Link. Looking for Chemicals which are hidden . BP Schools Link – . Creative Chromatography. 8. In some products the chemicals we want to measure can be hidden / masked by other bigger molecules. Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . Instrumentation. Invented by a Russian Botanist Mikhail . Tswett. in 1903 . He used chromatography . . to separate the colour . . pigments in plants from . . one another. Chromatography is a separation technique in which a mobile phase carrying a mixture moves in contact with a selectively adsorbent stationary phase. CLS 101: Chemistry For Nursing. Proteins Tests. 1. . Ninhydrin. Test to Detect Amino Acids. All . amino acids with free α-Amino . group will react with . ninhydrin. to give . deep . Blue-. purple. Mikhail . Tswett. , Russian, 1872-1919 . In 1906 . Tswett. used chromatography to separate plant pigments, He called the new technique . chromatography. . because the result of the analysis was . 'written colors” . Evidence. 1. Class characteristic—ink analysis, paper analysis, most handwriting analysis. 2. Individual characteristic--With document analysis, it is difficult to identify to an individual characteristic except with typewriter or printer specific marks.. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University. INTRODUCTION: GAS CHROMATOGRAPHY . Origin of gas chromatography: 1905, W. Ramsey . Separated mixture of gases and vapors . Used solid adsorbent: activated charcoal. . , All right reserved 123 International Journal of Agriculture Innovations and Research Volume 6 , Issue 1 , ISSN (Online) 2319 - 1473 Gas Chromatography : Principles , Advantages and Applications i pH Meter. pH Meter. What is pH Measurement?. pH Measurement specifies the degree of relative acidity or alkalinity of an aqueous solution at a given temperature. . It is generally measured using a pH Meter. . Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents.