PPT-Gas Chromatography Introduction, theory, instrumentation, Carrier Gas

1 Dr Nisha Sharma Associate Professor Pharmacy CSJM University INTRODUCTION GAS CHROMATOGRAPHY Origin of gas chromatography 1905 W Ramsey Separated mixture of gases and vapors Used solid adsorbent activated charcoal . Chapter 4. Best Broken into four categories. Theoretical Background. Gas Chromatography. HPLC. Quantitation, Calibration, Standardisation and Validation. Theory. Review of Partitioning. You need to be aware of the following concepts in order to have any idea about this chapter!. 10/9/2013. Ohio University - Dr. Cyders. 1. Engineering measurements are usually taken by some form of . transducer. . A transducer transforms one form of energy into another – an example would be a thermocouple, which converts a temperature differential to an . Gas Chromatograph. Gas chromatography (GC). , is a common type of . chromatography . used in . analytical . chemistry for separating and analyzing compounds that can be vaporized without decomposition. . Mikhail . Tswett. , Russian, 1872-1919 . In 1906 . Tswett. used chromatography to separate plant pigments, He called the new technique . chromatography. . because the result of the analysis was . 'written colors” . Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry A sample containing the materials to be separated is injected into the gas chromatograph. . Gas Chromatography. A mobile phase (carrier gas) moves through a column that contains a wall coated or granular solid coated stationary phase. . Gas Chromatography Mass Spectrometry Dr Kersti Karu email: kersti.karu@ucl.ac.uk Office number: Room LG 11 Recommended Textbooks: - “ Analytical Chemistry ” , G. D. Christian, P. K. Dasgupta , All right reserved 123 International Journal of Agriculture Innovations and Research Volume 6 , Issue 1 , ISSN (Online) 2319 - 1473 Gas Chromatography : Principles , Advantages and Applications i pH Meter. pH Meter. What is pH Measurement?. pH Measurement specifies the degree of relative acidity or alkalinity of an aqueous solution at a given temperature. . It is generally measured using a pH Meter. . Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. PRESENTED BY SAIMOUN. INTRODUCTION. . Parasite. :- A living organism which receives nourishment and shelter from another organism where it lives .. . Classes of parasites . :-. . a. . Ecto-parasite. derivatization. ,. Temperature programming, advantages, disadvantages and applications. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University. INTRODUCTION: GAS CHROMATOGRAPHY . Origin of gas chromatography: 1905, W. Ramsey . Introduction, theory, instrumentation, . derivatization. ,. Temperature programming, advantages, disadvantages and applications. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University.