PPT-Flash Chromatography

Hazards and Controls Flash Chromatography Hazard Control Spilled solvent could contact skin Solvent could penetrate through gloves and contact skin Wear goggles gloves nitrile and lab coat. hree month high Flash China M anufacturing Output Index at in Octo ber in September Five month low ata collected 13 21 Octo ber 2014 The HSBC Flash China Manufacturing Purchasing 0DQDJHUV5752657347QGH57484573475735530 is published on a monthly basi PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . AP Investigation 5: Photosynthesis – Part I. Chromatography. T. echnique . used to separate a mixture into its component . molecules. The . molecules migrate, or move up the paper, at different rates because of differences in solubility, molecular mass, and hydrogen bonding with the paper. BP Schools Link. Looking for Chemicals which are hidden . BP Schools Link – . Creative Chromatography. 8. In some products the chemicals we want to measure can be hidden / masked by other bigger molecules. Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Evidence. 1. Class characteristic—ink analysis, paper analysis, most handwriting analysis. 2. Individual characteristic--With document analysis, it is difficult to identify to an individual characteristic except with typewriter or printer specific marks.. BASICS OF CHROMATOGRAPHY Prepared by DR: Sherif Abdel- Naby Abdel- Gawad PhD Analytical Chemistry-Faculty of Pharmacy - Cairo University History of Chromatography Mikhail Tswett is credited with Lecture 7 a Chromatography I Introduction Chromatography was discovered by Russian botanist Mikhail Semyonovich Tsvett , who separated plant pigments using calcium carbonate columns (1901) Martin and Synge ( GC . is currently one of the most popular methods for separating and analyzing compounds. . GC can be applied to the separation of any compound that is either naturally volatile (i.e., readily goes into the gas phase) or can be converted to a volatile derivative.. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University. INTRODUCTION: GAS CHROMATOGRAPHY . Origin of gas chromatography: 1905, W. Ramsey . Separated mixture of gases and vapors . Used solid adsorbent: activated charcoal. . A sample containing the materials to be separated is injected into the gas chromatograph. . Gas Chromatography. A mobile phase (carrier gas) moves through a column that contains a wall coated or granular solid coated stationary phase. . Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . Dr . Debalina. . Basu. Department of Microbiology. Surendranath. College. Paper Chromatography. Principle. What is Descending Paper Chromatography?. Descending paper chromatography is an analytical technique in which the mobile phase moves downward through the stationary phase. In other words, in this method, the development of the paper occurs due to the movement of the solvent downwards on the paper. Therefore, the solvent reservoir should be at the top of the paper. In this process, the movement of the solvent is governed by gravity as well as capillary action.. Definition. Ion-exchange chromatography. (or . ion chromatography. ) is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. . 1975 by Small, Stevens and Baumann.