PPT-Chromatography TLC Thin layer chromatography

Stationary phase Mobile phase Separation AND Characterization Chromatography Basics Based on different affinities for stationary and mobile phases Silica gel polar watercovered surface Compounds. Supervisor:. Anton Zavialov. By: . Elham Barazeghi. Mehrafarin Ramezani. Affinity Chromatography:. Separation of proteins by reversible interaction of proteins and specific ligand in matrix of column. PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . Invention of Chromatography. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . AP Investigation 5: Photosynthesis – Part I. Chromatography. T. echnique . used to separate a mixture into its component . molecules. The . molecules migrate, or move up the paper, at different rates because of differences in solubility, molecular mass, and hydrogen bonding with the paper. Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . (last updated . June 2015). TLC Teams. Team 1. Team 2. Attending. Attending. Fellow. Fellow. Nurse practitioner. Nurse practitioner. 2 Residents. (Anesthesia. and EM). 2 Residents. (Internal medicine). Experiment 6. BCH 333. [practical]. Objectives:. -. Separation . of amino acids using paper . chromatography and TLC.. :. Chromatography. -is the collective term for a set of laboratory techniques . for separate and identify the components of a mixture. Instrumentation. Invented by a Russian Botanist Mikhail . Tswett. in 1903 . He used chromatography . . to separate the colour . . pigments in plants from . . one another. Chromatography is a separation technique in which a mobile phase carrying a mixture moves in contact with a selectively adsorbent stationary phase. (TLC). Thin layer chromatography (TLC) is an important technique for. identification. and . separation. of mixtures of organic compounds. It is useful in:. Identification of components of a mixture (using appropriate standards). coupling. Dr. s. . Francis Maquin (1) . . Pierre . Bernard . Savary. (2). . (1) Sanofi ; . centre. de . recherches. . ;. . 13 . Quai Jules . Guedes. ;. . 94430 Vitry . sur. . Seine ; France.. Lecture 7 a Chromatography I Introduction Chromatography was discovered by Russian botanist Mikhail Semyonovich Tsvett , who separated plant pigments using calcium carbonate columns (1901) Martin and Synge ( Benzhydrol. and . Benzophenone. by. Thin- Layer Chromatography . Biphenyl, . Benzhydrol. and . Benzophenone. . . Biphenyl. . Benzhydrol. . Benzophenone. Thin- Layer Chromatography. (TLC). . Botanist. In 1906 Tswett used to chromatography to separate plant pigments. He called the new technique chromatography because the result of the analysis was 'written in color' along the length of the adsorbent column. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents.