PPT-Chromatography TLC Thin layer chromatography

Stationary phase Mobile phase Separation AND Characterization Chromatography Basics Based on different affinities for stationary and mobile phases Silica gel polar watercovered surface Compounds. A little history. To write with colors -- literally translated from its Greek roots . chroma. . and . graphein. , chromatography was first developed by the Russian botanist Mikhail . Tswett. in 1903 as he produced a colorful separation of plant pigments through a column of calcium carbonate, particularly the . Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. Charcoal C Florisil MgO/SiO (anhydrous) Least Strongly Adsorbent The eluting solvent should also show a maximum of selectivity in its ability to dissolve or desorb the substances being separated. T The next two (2) experiments introduce Gas Chromatography and Simple & Fractional Distillation. This Week. Gas Chromatography – Acetates. Pavia – p. 817 - 836. Slayden – . p. 39 - 31. . Experiment 6. BCH 333. [practical]. Objectives:. -. Separation . of amino acids using paper . chromatography and TLC.. :. Chromatography. -is the collective term for a set of laboratory techniques . for separate and identify the components of a mixture. (TLC). Thin layer chromatography (TLC) is an important technique for. identification. and . separation. of mixtures of organic compounds. It is useful in:. Identification of components of a mixture (using appropriate standards). Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry 1- Adsorption chromatography.. 2- Partition chromatography.. 3- Ion exchange chromatography.. 4- Gel filtration chromatography.. 5- Affinity chromatography.. Thin layer chromatography (TLC). is a method for . . Chemistry. II. Gas. . Chromatography. Mobile . phase. : . inert. . gases. (. helium. , . nitrogen. , argon . etc. ). Stationary. . phases. : in . capillary. . column. Liquid (. siloxane. . polymers. . Senior Design Team sddec19-07. Olouwole Eteka,Kossi Egla, Zheyuan Tang,Ugerah Abalu,Hao Wang,Yifu Zhang. Advisor: Dr Meng Lu. Dr.Methak. A . ljboori. Ion Exchange Chromatography. Ion exchange chromatography (IEX) is a technique that is commonly used in . biomolecule. purification. It involves the separation of molecules on the basis of their charge. . Botanist. In 1906 Tswett used to chromatography to separate plant pigments. He called the new technique chromatography because the result of the analysis was 'written in color' along the length of the adsorbent column. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents.