PPT-Lecture 7 a Chromatography

Lecture 7 a Chromatography I Introduction Chromatography was discovered by Russian botanist Mikhail Semyonovich Tsvett who separated plant pigments using calcium carbonate columns 1901 Martin and Synge . PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . Announcements I. Exam 2 – Monday. Covering Ch. 6 (topics since exam 1), 7, 8-1, 17, and parts of 22 (up to and including retention and retention factor). Will review topics today. Help Session – Friday 11 to 12, Sequoia 446. The next two (2) experiments introduce Gas Chromatography and Simple & Fractional Distillation. This Week. Gas Chromatography – Acetates. Pavia – p. 817 - 836. Slayden – . p. 39 - 31. . Announcements I. Quiz 4. Today. Syllabus error (Chromatography chapter = 23 – not 22). Formal . Lab Report Information. Posted online (class website). Parts A (Introduction and Methods) and B (Results added. Introduction. Gas chromatography is used in many research labs, industrial labs (quality control), forensic (arson and drug analysis, toxicology, etc.), environmental labs (water, soil, air), and even in the popular TV culture (crime shows like NCIS (. Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry . Chemistry. II. Gas. . Chromatography. Mobile . phase. : . inert. . gases. (. helium. , . nitrogen. , argon . etc. ). Stationary. . phases. : in . capillary. . column. Liquid (. siloxane. . polymers. Announcements I. Still grading last homework and make up quiz. Posting solutions of these (soon). Final Exam. Thursday, May 18. th. 12:45-2:45. About 50% Review/50% New Material. Allowed 1 8.5” x 11” sheet of notes (no equations provided). Announcements I. Homework Set 3 – due today. Quiz 5 (Ave on Q5 was 1.75. ) . –. Final . Exam. Thursday, May 18. th. 12:45-2:45. About 50% Review/50% New Material. Allowed 1 8.5” x 11” sheet of notes (no equations provided). Dr.Methak. A . ljboori. Ion Exchange Chromatography. Ion exchange chromatography (IEX) is a technique that is commonly used in . biomolecule. purification. It involves the separation of molecules on the basis of their charge. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . By . Dr. . Amjad. M. T. Khan. Assistant Professor. Department of Chemistry. AMU, Aligarh. CONTENTS. Introduction to chromatography. History. Principles. Importance. Chromatographic terms. Classification of chromatography. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Announcements I. AA . Lab . – Due today. Quiz 4. On Wednesday. On Acids part of Ch. 6/Chapter 7 materials/Chapter 18 materials (extractions). Today’s Lecture. Chapter . 18 . – Spectroscopy. Beer’s Law.