PPT-BASICS OF CHROMATOGRAPHY Prepared by DR: Sherif Abdel- Naby

BASICS OF CHROMATOGRAPHY Prepared by DR Sherif Abdel Naby Abdel Gawad PhD Analytical ChemistryFaculty of Pharmacy Cairo University History of Chromatography Mikhail Tswett is credited with. PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . Invention of Chromatography. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . Chapter 4. Best Broken into four categories. Theoretical Background. Gas Chromatography. HPLC. Quantitation, Calibration, Standardisation and Validation. Theory. Review of Partitioning. You need to be aware of the following concepts in order to have any idea about this chapter!. Participants and basic procedure?. 10-12 year old boys. Naturally formed groups (e.g., friendships developed). Boys separated into 2 groups (e.g., Rattlers) . Sherif (cont.). ~ Group Development . ~. The next two (2) experiments introduce Gas Chromatography and Simple & Fractional Distillation. This Week. Gas Chromatography – Acetates. Pavia – p. 817 - 836. Slayden – . p. 39 - 31. . Sherif (1954) Robber’s Cave Study. Sherif Robber’s Cave Study QUIZ . We have already watched a video and made brief notes on the study. Test . your knowledge – Individual task but we shall discuss each answer . Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry . Chemistry. II. Gas. . Chromatography. Mobile . phase. : . inert. . gases. (. helium. , . nitrogen. , argon . etc. ). Stationary. . phases. : in . capillary. . column. Liquid (. siloxane. . polymers. Page 1 of 7 YINA WU Doctor of Philosophy, Civil Engineering (Transportation), University of Central Florida, Florida, USA December 2017 Dissertation: Improving Safety under Reduced Visibility Bas Abdel Halim Hafez was an Egyptian singer and actor born in 1929 in Cairo Hafez146s musical ability was evident at an early age His elder brother Ismail who became a public-school music teacher encoura Dr.Methak. A . ljboori. Ion Exchange Chromatography. Ion exchange chromatography (IEX) is a technique that is commonly used in . biomolecule. purification. It involves the separation of molecules on the basis of their charge. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Definition. Ion-exchange chromatography. (or . ion chromatography. ) is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. . 1975 by Small, Stevens and Baumann.