PPT-Paper and Thin Layer Chromatography (TLC)

Experiment 6 BCH 333 practical Objectives Separation of amino acids using paper chromatography and TLC Chromatography is the collective term for a set of laboratory techniques for separate and identify the components of a mixture. A little history. To write with colors -- literally translated from its Greek roots . chroma. . and . graphein. , chromatography was first developed by the Russian botanist Mikhail . Tswett. in 1903 as he produced a colorful separation of plant pigments through a column of calcium carbonate, particularly the . Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. Charcoal C Florisil MgO/SiO (anhydrous) Least Strongly Adsorbent The eluting solvent should also show a maximum of selectivity in its ability to dissolve or desorb the substances being separated. T Chromatography. Chromatography is a technique of separation of different dissolved constituents of a mixture by absorbing them over an appropriate adsorbent material .It is a versatile method of separating different kinds of chemical mixtures .. (TLC). Thin layer chromatography (TLC) is an important technique for. identification. and . separation. of mixtures of organic compounds. It is useful in:. Identification of components of a mixture (using appropriate standards). Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Mikhail . Tswett. , Russian, 1872-1919 . In 1906 . Tswett. used chromatography to separate plant pigments, He called the new technique . chromatography. . because the result of the analysis was . 'written colors” . Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry 1- Adsorption chromatography.. 2- Partition chromatography.. 3- Ion exchange chromatography.. 4- Gel filtration chromatography.. 5- Affinity chromatography.. Thin layer chromatography (TLC). is a method for . . Senior Design Team sddec19-07. Olouwole Eteka,Kossi Egla, Zheyuan Tang,Ugerah Abalu,Hao Wang,Yifu Zhang. Advisor: Dr Meng Lu. Chemistry 1060 Laboratory. Analysis. Purity determination. Thin Layer Chromatography (TLC). Separation and identification. stationary . phase – silica gel. mobile . phase – organic solvent. Relative attraction to the two phases. Introduction, Principle, Methodology, . Rf. values, advantages, disadvantages and applications. Dr. . Nisha. Sharma, . Associate Professor . School of Pharmaceutical Sciences. C.S.J.M. University, Kanpur. . Botanist. In 1906 Tswett used to chromatography to separate plant pigments. He called the new technique chromatography because the result of the analysis was 'written in color' along the length of the adsorbent column. The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures.