PPT-Affinity Chromatography

Supervisor Anton Zavialov By Elham Barazeghi Mehrafarin Ramezani Affinity Chromatography Separation of proteins by reversible interaction of proteins and specific ligand in matrix of column. Revised October, . 2013. This Department of Veterans Affairs Training Series on Affinity Groups for Veterans and . military service members . is designed to help organizations understand the benefits to having Veteran and military affinity groups and the practices that could be implemented to make them more effective.. Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. Chapter 4. Best Broken into four categories. Theoretical Background. Gas Chromatography. HPLC. Quantitation, Calibration, Standardisation and Validation. Theory. Review of Partitioning. You need to be aware of the following concepts in order to have any idea about this chapter!. BP Schools Link. Looking for Chemicals which are hidden . BP Schools Link – . Creative Chromatography. 8. In some products the chemicals we want to measure can be hidden / masked by other bigger molecules. The next two (2) experiments introduce Gas Chromatography and Simple & Fractional Distillation. This Week. Gas Chromatography – Acetates. Pavia – p. 817 - 836. Slayden – . p. 39 - 31. . Principle and Definition. Principle. The principle of chromatographic separation is very simple. The process is achieved by . distributing the substances. to be separated between a . mobile phase. and a . Instrumentation. Invented by a Russian Botanist Mikhail . Tswett. in 1903 . He used chromatography . . to separate the colour . . pigments in plants from . . one another. Chromatography is a separation technique in which a mobile phase carrying a mixture moves in contact with a selectively adsorbent stationary phase. Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Mikhail . Tswett. , Russian, 1872-1919 . In 1906 . Tswett. used chromatography to separate plant pigments, He called the new technique . chromatography. . because the result of the analysis was . 'written colors” . ASSISTANT PROFESSOR. PG&RESEARCH DEPARTMENT OF BIOTECHNOLOGY. BONSECOURS COLLEGE FOR WOMEN. THANJAVUR. WHAT IS THE . DOWNSTREAM . PROCESSING. Downstream processing refers to the recovery and purification of biosynthetic products, particularly pharmaceuticals, from natural sources such as animal or plant tissue or fermentation broth, including the recycling of salvageable components and the proper treatment and disposal of waste. It is an essential step in the manufacture of pharmaceuticals such as antibiotics, hormones (e.g. insulin and humans growth hormone), antibodies (e.g. infliximab . CPSC 481 Tutorial 2. 1. Anthony Tang, with acknowledgements to Julie . Kientz. Agenda. Presentation by TA: . 5 . mins. Brainstorm: 5 . mins. Affinity diagramming: 10 . mins. Sketching . a prototype: 30 . 1. Ankur Garg. Sunav Choudhary. Payal Bajaj.  . Adobe Research.  Adobe Research.  Stanford University. Sweta Agrawal. Abhishek Kedia. Shubham Agrawal. Adobe. Adobe. Adobe. What is Geo-fencing?. 2. Dr . Debalina. . Basu. Department of Microbiology. Surendranath. College. Paper Chromatography. Principle. What is Descending Paper Chromatography?. Descending paper chromatography is an analytical technique in which the mobile phase moves downward through the stationary phase. In other words, in this method, the development of the paper occurs due to the movement of the solvent downwards on the paper. Therefore, the solvent reservoir should be at the top of the paper. In this process, the movement of the solvent is governed by gravity as well as capillary action.. Purposes of Affinity Groups (Employee Resource Groups). Affinity Groups (AG). create an open forum for staff who share common interests/concerns to meet and support one another in creatively addressing those concerns .