PPT-UNIT –III Thin layer chromatography

Introduction Principle Methodology Rf values advantages disadvantages and applications Dr Nisha Sharma Associate Professor School of Pharmaceutical Sciences CSJM University Kanpur. Supervisor:. Anton Zavialov. By: . Elham Barazeghi. Mehrafarin Ramezani. Affinity Chromatography:. Separation of proteins by reversible interaction of proteins and specific ligand in matrix of column. A little history. To write with colors -- literally translated from its Greek roots . chroma. . and . graphein. , chromatography was first developed by the Russian botanist Mikhail . Tswett. in 1903 as he produced a colorful separation of plant pigments through a column of calcium carbonate, particularly the . Prepared by :. Mona . Abo. . Hasera. Chromatography. First application by M. S. . Tswett. 1903. For the separation of plant pigments. Since the components had different colors the Greek . chromatos. Chapter 4. Best Broken into four categories. Theoretical Background. Gas Chromatography. HPLC. Quantitation, Calibration, Standardisation and Validation. Theory. Review of Partitioning. You need to be aware of the following concepts in order to have any idea about this chapter!. Hazards and Controls. Flash Chromatography. Hazard . Control. Spilled solvent could contact skin. Solvent could penetrate through gloves and contact skin . Wear goggles, gloves (nitrile), and lab coat. (TLC). Thin layer chromatography (TLC) is an important technique for. identification. and . separation. of mixtures of organic compounds. It is useful in:. Identification of components of a mixture (using appropriate standards). Stationary phase. Mobile phase. Separation AND Characterization. Chromatography Basics. Based on different affinities for stationary and mobile phases. Silica gel: polar, water-covered surface. Compound(s). Evidence. 1. Class characteristic—ink analysis, paper analysis, most handwriting analysis. 2. Individual characteristic--With document analysis, it is difficult to identify to an individual characteristic except with typewriter or printer specific marks.. Lecture 7 a Chromatography I Introduction Chromatography was discovered by Russian botanist Mikhail Semyonovich Tsvett , who separated plant pigments using calcium carbonate columns (1901) Martin and Synge ( . Chemistry. II. Gas. . Chromatography. Mobile . phase. : . inert. . gases. (. helium. , . nitrogen. , argon . etc. ). Stationary. . phases. : in . capillary. . column. Liquid (. siloxane. . polymers. . Senior Design Team sddec19-07. Olouwole Eteka,Kossi Egla, Zheyuan Tang,Ugerah Abalu,Hao Wang,Yifu Zhang. Advisor: Dr Meng Lu. 1. Dr. Nisha Sharma, Associate Professor, Pharmacy, C.S.J.M. University. INTRODUCTION: GAS CHROMATOGRAPHY . Origin of gas chromatography: 1905, W. Ramsey . Separated mixture of gases and vapors . Used solid adsorbent: activated charcoal. . Dr.Methak. A . ljboori. Ion Exchange Chromatography. Ion exchange chromatography (IEX) is a technique that is commonly used in . biomolecule. purification. It involves the separation of molecules on the basis of their charge. . Botanist. In 1906 Tswett used to chromatography to separate plant pigments. He called the new technique chromatography because the result of the analysis was 'written in color' along the length of the adsorbent column.