PPT-Bioseparation Lab7 Ion Exchange Chromatography

DrMethak A ljboori Ion Exchange Chromatography Ion exchange chromatography IEX is a technique that is commonly used in biomolecule purification It involves the separation of molecules on the basis of their charge. PREPARED BY-. MD.MARUF HASSAN. Chromatography. The process or technique of separating molecules or components in a mixture . solution (gas or . liquid) . according . to the differential absorption and elution . Course: Methods in protein chemistry. Rahman M. . Mahfuz. ur. 2012/01/11. SLU. To seperate a . particular. protin from all other proteins and cell . components. . There are many types of proteins within an ogranism . Dr. . Kamal. E. M. . Elkahlout. Chapter 1. An overview. SEPARATION TECHNIQUES. Separation of one or more components from a complex mixture is a requirement . for many . operations in . biotechnology industries.. Chromatography. Chromatography is a technique of separation of different dissolved constituents of a mixture by absorbing them over an appropriate adsorbent material .It is a versatile method of separating different kinds of chemical mixtures .. HPTLC 2017 Berlin. Yanhui. (Celia) Wang and Susan V. Olesik. Department of Chemistry and Biochemistry. Ohio State University. Columbus, Ohio 43016. Areas where UTLC separations of amino acids, peptides and proteins may be valuable. Dr. Kevin Ahern. Protein Purification. Applications of Biochemistry Knowledge. Opening Cells. Centrifugation. Fractionation. Dialysis. Applications of Biochemistry Knowledge. Separates salts from proteins. ion . exchange chromatography. BCH 333[practical]. Objectives. :. 1- The objective of this experiment is for students to learn the principles of ion exchange chromatography by separating the charged molecules using a salt gradient.. Instrumental Analysis Instrumentation Paper Chromatography GC – Gas Chromatography HPLC- High Performance Liquid Chromatography IR – Infrared Spectroscopy UV-Vis Spectroscopy MS – Mass Spectrometry . Chemistry. II. Gas. . Chromatography. Mobile . phase. : . inert. . gases. (. helium. , . nitrogen. , argon . etc. ). Stationary. . phases. : in . capillary. . column. Liquid (. siloxane. . polymers. Mikhail Tswett. Russian Botanist. (1872-1919). . Mikhail Tswett. invented chromatography in 1901 during his . research on plant pigments. . . He used the technique to separate various plant pigments such as . The word Chromatography is derived fro two Greek . words –. Chroma . means – colour and . graphein. . to write. . Chromatography is the collective term for a family of laboratory techniques for the separation of mixtures. A. . technique for the separation of a mixture by passing it in solution or suspension through a medium in which the components move at . different . rates. .. Principle. It is based on the principle of the partition of the solute between two phases/solvents. Definition. Ion-exchange chromatography. (or . ion chromatography. ) is a process that allows the separation of ions and polar molecules based on the charge properties of the molecules. . 1975 by Small, Stevens and Baumann. Ion Exchange Chromatography. BCH 333[practical]. Objectives:. 1- The objective of this experiment is for students to learn the principles of ion exchange chromatography by separating the charged molecules using buffer and salt..