PPT-Day 2: Microscope Skills

Learning Targets I will learn about the proper care of a compound microscope I will be able to prepare my own slide wet mount I will learn how to focus on a specimen using each of the lenses. Microscopes are delicate and expensive instruments they should be handled with utmost care Before you use the microscope your instructor will explain its proper use Following are rule s that will protect the microscope and insure that you can make m We always offer our customers the best quality for the lowest price. We pride ourselves on providing high quality products, unbeatable low prices, huge selections, and personalized customer service. We always offer our customers the best quality for the lowest price. We pride ourselves on providing high quality products, unbeatable low prices, huge selections, and personalized customer service. POLARISED LIGHT MICROSCOPE . Designed to observe optically anisotropic specimen (BIREFRINGENCE SPECIMEN). Equipped with POLARISER and ANALYZER . Works on the principle of INTERFERENCE . COMPONENTS OF POLARISED . Basics. Nosepiece. Objectives. Stage Clips. Light. Ocular lens. (Eyepiece). Arm. Stage. Coarse . Adjustment. Knob. Fine . Adjustment. Knob. Always carry a microscope with one hand holding the arm and one hand under the base.. Introduction to the Microscope. Types of . Microscopes. Calibration. Focusing. Types of Microscopes. Light Microscope - . Ocular lens on eyepiece and objective lens on turret multiply magnification.. Basics. By: Kaitlyn Schroeder; ETEAMS 2014. Modified from . Trimpe. . 2005 http://sciencespot.net/. 1. Microscope Basics . Uncover the microscope in front of you and follow along with the lecture. . . 2. Compound Light Microscope. Used to observe small objects. Magnifies images up to 2000X their size. 3. Different parts of a microscope. copyright cmassengale. SCI.9-12.B-1.2. - [Indicator] - Use appropriate laboratory apparatuses, technology, and techniques safely and accurately when conducting a scientific investigation. http://biology.unm.edu/ccouncil/Biology_203/Summaries/Microscopes.htm. 1. When moving your microscope, always carry it with both hands. Grasp the arm with one hand and place the other hand under the base for support.. How to Focus on Specimen. Turn on light source on high. Turn the objective to low (4x). Lower the objective lens so that it is close but not touching the slide. Center the image by moving the stage. Adjust the diaphragm for optimum light. Light Microscope. Simple – uses a single lens. Compound – uses a set of lenses or lens systems. Simple Light Microscope. Compound Microscope. Mechanical Parts. Used to support and adjust the parts. Light Microscope. Simple – uses a single lens. . Compound – uses a set of lenses or lens systems (this is the type of microscope we use in class, it has the eyepiece lens (ocular) and the objective lens). Compound Light Microscope. The compound light microscope is a common tool in a life science laboratory. A . compound light microscope. is an instrument that magnifies small objects so that they can be seen easily. . Depth of . f. ield: small. Magnification. Uses lenses arranged in series, made of optical material that bends light. All magnification takes place via lenses. Uses . diascopic. illumination (that is, light is transmitted through the specimen). Magnification up to about 1000x..